Quantitative analysis of glycans, related genes, and proteins in two human bone marrow stromal cell lines using an integrated strategy.
Quantitative analysis of glycans, related genes, and proteins in two human bone marrow stromal cell lines using an integrated strategy.
复制标题
使用集成策略对两种人骨髓基质细胞系中的聚糖、相关基因和蛋白质进行定量分析
DOI：
10.1016/j.exphem.2015.04.009
复制
发表时间：
2015-09
影响因子：
2.6
通讯作者：
Guan F
中科院分区：
文献类型：
--
作者：
Li X;Li D;Pang X;Yang G;Deeg HJ;Guan F
聚糖表达的改变与骨髓微环境中的细胞-细胞信号转导和细胞功能调节有关。这种微环境的研究通常使用两种人骨髓基质细胞系，HS 5和HS 27 a，与骨髓细胞共培养。我们假设这两种细胞系之间的差异蛋白糖基化可能有助于在体外共培养模型中的功能差异。在这项研究中，我们采用了一种综合策略，使用基因组学，蛋白质组学和功能性糖组学技术的全局表达谱的N-聚糖及其相关基因和酶在HS 5与HS 27 a细胞。HS 5细胞显示出显著增强的平分N-聚糖水平（由MGAT 3催化），而HS 27 a细胞显示出增强的Galβ1，4GlcNAc水平（由β 4GalT 1催化）。这种综合策略提供了有用的信息，聚糖及其相关的糖基和糖基转移酶在骨髓微环境中的功能作用，并为今后的研究基质细胞和骨髓瘤细胞之间的串扰共培养的基础。
Altered expressions of glycans is associated with cell-cell signal transduction and regulation of cell functions in the bone marrow microenvironment. Studies of this microenvironment often use two human bone marrow stromal cell lines, HS5 and HS27a, co-cultured with myeloid cells. We hypothesized that differential protein glycosylation between these two cell lines may contribute to functional differences in in vitro co-culture models. In this study, we applied an integrated strategy using genomic, proteomic, and functional glycomic techniques for global expression profiling of N-glycans and their related genes and enzymes in HS5 vs. HS27a cells. HS5 cells showed significantly enhanced levels of bisecting N-glycans (catalyzed by MGAT3), whereas HS27a cells showed enhanced levels of Galβ1, 4GlcNAc (catalyzed by β4GalT1). This integrated strategy provides useful information regarding the functional roles of glycans and their related glycogenes and glycosyltransferases in the bone marrow microenvironment, and a basis for future studies of crosstalk among stromal cells and myeloma cells in co-culture.