TRANSLATION INITIATION AND RIBOSOMAL BIOGENESIS - INVOLVEMENT OF A PUTATIVE RIBOSOMAL-RNA HELICASE AND RPL46
TRANSLATION INITIATION AND RIBOSOMAL BIOGENESIS - INVOLVEMENT OF A PUTATIVE RIBOSOMAL-RNA HELICASE AND RPL46
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DOI：
10.1126/science.2408148
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发表时间：
1990-03-02
期刊：
影响因子：
56.9
通讯作者：
DAVIS, RW
中科院分区：
文献类型：
--
作者：
SACHS, AB;DAVIS, RW
酿酒酵母SPB基因（spb 1-spb 7）中的冷敏感突变抑制了由多聚腺苷酸结合蛋白基因（PAB 1）缺失引起的翻译起始抑制。SPB 4蛋白属于三磷酸腺苷（ATP）依赖性RNA解旋酶家族。在spb 4 -1突变体中发生的25 S核糖体RNA（rRNA）的异常产生或SPB 2（RPL 46）的缺失允许PAB 1的缺失。这些数据表明，影响60 S亚基形成的不同步骤的突变可以允许PAB独立的翻译，并且它们表明spb突变的进一步表征可以深入了解核糖体的生物起源。
Cold-sensitive mutations in the SPB genes (spb1-spb7) of Saccharomyces cerevisiae suppress the inhibition of translation initiation resulting from deletion of the poly(A)-/binding protein gene (PAB1). The SPB4 protein belongs to a family of adenosine triphosphate (ATP)-dependent RNA helicases. The aberrant production of 25S ribosomal RNA (rRNA) occurring in spb4-1 mutants or the deletion of SPB2 (RPL46) permits the deletion of PAB1. These data suggest that mutations affecting different steps of 60S subunit formation can allow PAB-independent translation, and they indicate that further characterization of the spb mutations could lend insight into the biogenesis of the ribosome.