SPATIAL-DISTRIBUTION OF THE CAPACITY TO INITIATE A SECONDARY EMBRYO IN THE 32-CELL EMBRYO OF XENOPUS-LAEVIS
SPATIAL-DISTRIBUTION OF THE CAPACITY TO INITIATE A SECONDARY EMBRYO IN THE 32-CELL EMBRYO OF XENOPUS-LAEVIS
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DOI：
10.1016/0012-1606(90)90365-p
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发表时间：
1990-12-01
影响因子：
2.7
通讯作者：
KAGEURA, H
中科院分区：
文献类型：
--
作者：
KAGEURA, H
为了研究非洲爪哇早期胚胎中背侧决定因素的空间分布，将32-细胞胚胎中的单个细胞移植到同步受体腹侧的同一层。他们启动次级胚胎的能力是通过次级胚胎的发生率和次级轴相对于初级胚胎的长度来衡量的。这种能力定位于最背柱的所有细胞(A1、B1、C1和D1)以及背外侧柱的植物细胞(C2和D2)。次生胚胎的发生率最高，次生胚胎的平均相对长度也最大。有效性按顺序递减：D1、B1、D2、C2和A1。将这些结果与32-细胞胚胎的Dale和Slack的S命运图相比较，我们得出结论：背侧决定因素的分布是唯一的，与任何组织的预期区域不一致，尽管它与背侧内胚层或脊索的预期区域有些相似.从这些结果来看，背侧决定因素似乎并不决定胚胎中的特定组织，而是决定胚胎的“背侧”区域.
To examine the spatial distribution of dorsal determinants in the early embryos of Xenopus laevis, individual cells from the 32-cell embryo were transplanted into the same tier of the ventral side of a synchronous recipient. Their abilities to initiate a secondary embryo were measured by the incidence of secondary embryos and by the length of the secondary axis relative to the primary embryo. The ability was found to be localized in all cells (A1, B1, C1, and D1) of the dorsal most column and in the vegetal cells (C2 and D2) of the dorsolateral column. Transplanted C1 (subequatorial) cells caused the highest incidence of a secondary embryo and the average relative length of the secondary embryo was also greatest. Effectiveness decreased in the order: D1, B1, D2, C2, and A1. When these results were compared with Dale and Slack''s fate map of the 32-cell embryo, it was concluded that the distribution of dorsal determinants is unique and does not coincide with the prospective regions for any tissues, though it is somewhat similar to the prospective region of dorsal endoderm or notochord. From these results it seems that dorsal determinants do not determine a particular tissue in an embryo but rather the "dorsal" region of an embryo.