Nucleotide-evoked ion transport and [Ca2+]i changes in normal and hyperhidrotic human sweat gland cells
Nucleotide-evoked ion transport and [Ca2+]i changes in normal and hyperhidrotic human sweat gland cells
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DOI：
10.1016/s0014-2999(00)00570-7
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发表时间：
2000-09-01
影响因子：
5
通讯作者：
Ko, WH
中科院分区：
文献类型：
--
作者：
Bovell, DL;Clunes, MT;Ko, WH
ATP和UTP的顶端和基底侧应用诱发[Ca2+](i)和短路电流（Isc）增加，正常和多汗症人分泌汗腺细胞在渗透性支持下生长为功能性极化上皮。基底外侧应用于多汗症细胞显示Ise明显高于正常细胞。当用thapsigargin预处理时，多汗症细胞也表现出与正常细胞在[Ca2+](i)和Isc对ATP的反应上的差异。数据表明，存在顶端和基底侧受体，允许核苷酸增加[Ca2+](i)和Isc。结果表明，从正常的上皮离子转运的变化有助于多汗症汗腺的特征性的过多的液体生产。(C) 2000 Elsevier Science B.V.版权所有
Apical and basolateral application of ATP and UTP evoked [Ca2+](i) and short circuit current (Isc) increases in normal and hyperhidrotic human eccrine sweat gland cells grown into functionally polarised epithelia on permeable supports. Basolateral application to hyperhidrotic cells exhibited a markedly greater increase in Ise than in normal cells. Hyperhidrotic cells also demonstrated differences from the normal in [Ca2+](i) and Isc responses to ATP when pre-treated with thapsigargin. The data demonstrate the presence of apical and basolateral receptors that allow nucleotides to increase [Ca2+](i) and Isc. The results suggest that changes from the normal in transepithelial ion transport contribute to the characteristic excessive fluid production of hyperhidrotic sweat glands. (C) 2000 Elsevier Science B.V. All rights reserved.