Qualitative and quantitative characterization of monomers of uncured bulk-fill and conventional resin-composites using liquid chromatography/mass spectrometry
Qualitative and quantitative characterization of monomers of uncured bulk-fill and conventional resin-composites using liquid chromatography/mass spectrometry
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DOI：
10.1016/j.dental.2015.03.010
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发表时间：
2015-06-01
期刊：
影响因子：
5
通讯作者：
Silikas, Nick
中科院分区：
文献类型：
--
作者：
Alshali, Ruwaida Z.;Salim, Nesreen A.;Silikas, Nick
目标.本研究的目的是使用反相液相色谱法结合电子喷雾电离质谱法评估所选散装填充和常规树脂复合材料的树脂基质单体组成。测试了6种散装填充（SureFil SDR、Venus Bulk Fill、X-tra base、Filtek Bulk Fill flowable、Sonic Fill和Tetric EvoCeram Bulk Fill）和8种常规树脂复合材料（Grandioso Flow、Venus Diamond Flow、X-Flow、Filtek Supreme XTE、Grandioso、Venus Diamond、TPH Spectrum和Filtek Z250）。为了评估树脂组合物和相对单体量，用反相液相色谱/电子喷雾电离质谱法分析未固化的树脂复合材料。计算检测到的化合物的logP值（疏水性的量度），并探索它们与反相液相色谱保留时间的相关性。资料分析采用单因素方差分析、Tukey事后检验、Pearson相关分析和回归分析，α = 0.05。检测到的主要单体是BisGMA、UDMA、TEGDMA和BisEMA。在不同材料中检测到可变组合的单体，其相对量显著不同。在Grandioso flow、X-flow、Venus Diamond和SureFilSDR中分别检测到其他单体，包括HDDMA、DEGDMA、TCD-DI-HEA和SDR-UDMA。logP与反相液相色谱保留时间呈正相关（r（2）= 0.62，p = 0.004）。除SureFil SDR外，整体填充树脂复合材料的树脂成分与传统材料相当。牙科单体的相对疏水性可以通过其反相液相色谱保留时间来确定。(C)2015年牙科材料学院。由爱思唯尔有限公司出版。保留所有权利。
Objectives. The aim of this study was to assess the resin matrix monomer composition of selected bulk-fill and conventional resin-composite materials using reverse phase liquid chromatography coupled with electron spray ionization mass spectrometry.Material and methods. Six bulk-fill (SureFil SDR, Venus Bulk Fill, X-tra base, Filtek Bulk Fill flowable, Sonic Fill, and Tetric EvoCeram Bulk Fill) and eight conventional resin-composites (Grandioso Flow, Venus Diamond Flow, X-Flow, Filtek Supreme XTE, Grandioso, Venus Diamond, TPH Spectrum, and Filtek Z250) were tested. For assessment of resin composition and relative monomer amounts, uncured resin-composites were analysed with reverse phase liquid chromatography/electron spray ionization mass spectrometry. logP values (a measure of hydrophobicity) of detected compounds were calculated and their correlation to reverse phase liquid chromatography retention time was explored. Data were analysed with one-way ANOVA, Tukey post hoc test, Pearson correlation and regression analyses at alpha=0.05.Results. The main monomers detected were BisGMA, UDMA, TEGDMA, and BisEMA. Monomers were detected at variable combinations in different materials with significantly different relative amounts. Other monomers were detected including HDDMA, DEGDMA, TCD-DI-HEA, and SDR-UDMA in Grandioso flow, X-flow, Venus Diamond, and SureFil SDR respectively. A positive correlation between logP and reverse phase liquid chromatography retention time was detected (r(2) = 0.62, p = 0.004).Conclusions. Resin composition of bulk-fill resin-composites is comparable to that of conventional materials with the exception of SureFil SDR. The relative hydrophobicity of dental monomers can be determined by their reverse phase liquid chromatography retention time. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.