Petrol and diesel exhaust particles accelerate the horizontal transfer of plasmid-mediated antimicrobial resistance genes
Petrol and diesel exhaust particles accelerate the horizontal transfer of plasmid-mediated antimicrobial resistance genes
复制标题
汽油和柴油尾气颗粒加速了质粒介导的抗菌素耐药基因的水平转移
DOI：
10.1016/j.envint.2018.02.038
复制
发表时间：
2018
影响因子：
11.8
通讯作者：
Chen Jianmin
中科院分区：
文献类型：
--
作者：
Zhang Ye;Gu April Z;Cen Tianyu;Li Xiangyang;Li Dan;Chen Jianmin
汽油和柴油消耗排放的颗粒是城市空气污染的主要组成部分，可通过直接吸入或通过大气沉积到水和土壤中的其他途径暴露于人类。抗微生物药物耐药性是现代卫生保健面临的最严重威胁之一。然而，汽油和柴油废气颗粒如何影响抗菌素耐药性基因（ARGs）在各种环境中的发展和传播，在很大程度上仍然未知。本文研究了97辛烷值汽油、93辛烷值汽油、轻柴油和船用重柴油4种具有代表性的汽油和柴油尾气颗粒对两种机会性大肠杆菌(E。杆菌菌株,E。coliS17-1（供体）和e。coliK12(接受者)。结果表明，与对照组相比，这四种具有代表性的纳米级颗粒诱导的共轭转移率呈浓度依赖性增加。研究还确定了ARGs加速转移的潜在机制，包括细胞内活性氧（ROS）的产生及其引起的氧化应激、SOS反应、细胞形态的改变、膜蛋白基因mRNA表达的改变以及促进共轭转移的相关基因的表达。这些发现为汽油和柴油尾气颗粒带来的抗菌素耐药性风险提供了新的证据和机制见解，并强调了对替代燃料采取严格战略以减轻空气污染和健康风险的影响和必要性。
Particles exhausted from petrol and diesel consumptions are major components of urban air pollution that can be exposed to human via direct inhalation or other routes due to atmospheric deposition into water and soil. Antimicrobial resistance is one of the most serious threats to modern health care. However, how the petrol and diesel exhaust particles affect the development and spread of antimicrobial resistance genes (ARGs) in various environments remain largely unknown. This study investigated the effects and potential mechanisms of four representative petrol and diesel exhaust particles, namely 97 octane petrol, 93 octane petrol, light diesel oil, and marine heavy diesel oil, on the horizontal transfer of ARGs between two opportunisticEscherichia coli(E. coli) strains,E. coliS17-1 (donor) andE. coliK12 (recipient). The results demonstrated that these four representative types of nano-scale particles induced concentration-dependent increases in conjugative transfer rates compared with the controls. The underlying mechanisms involved in the accelerated transfer of ARGs were also identified, including the generation of intracellular reactive oxygen species (ROS) and the consequent induction of oxidative stress, SOS response, changes in cell morphology, and the altered mRNA expression of membrane protein genes and those involved in the promotion of conjugative transfer. The findings provide new evidences and mechanistic insights into the antimicrobial resistance risks posed by petrol and diesel exhaust particles, and highlight the implications and need for stringent strategies on alternative fuels to mitigate air pollution and health risks.