Adventitious root primordia formation and development in the stem of Ananas comosus var. bracteatus slip
Adventitious root primordia formation and development in the stem of Ananas comosus var. bracteatus slip
复制标题
菠萝茎中不定根原基的形成和发育。
DOI：
10.1080/15592324.2021.1949147
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发表时间：
2021-07-22
影响因子：
2.9
通讯作者：
Ma, Jun
中科院分区：
文献类型：
--
作者：
Owusu Adjei, Mark;Xiang, Yixuan;Ma, Jun
一个果实上约有4-6个片，是凤梨有效再生的良好材料。苞片。不定根 (AR) 诱导对于 Ananas comosus var. 的繁殖至关重要。苞片滑落。生长调节剂处理和培养基是影响滑条生长和生根的重要因素。为了筛选最佳的滑生根方法并揭示滑生根的解剖过程，本研究通过生长调节剂、培养基的不同处理诱导滑生根，并通过石蜡切片观察滑茎结构、AR起源和形成过程。结果表明，插条用100 mg/L氨基苯并三唑（ABT）处理6 h，在河沙：椰壳：园土2：2：1培养基中培养是最佳生根方法。适当补充ABT可提高可溶性糖含量、可溶性蛋白质含量、多酚氧化酶（PPO）活性和过氧化物酶（POD）酶活性，从而提高生根能力。滑茎的结构与其他单子叶植物有很大不同，它由表皮、皮层和中柱组成，维管组织分布在皮层和中柱中。 AR原基起源于位于皮质和中柱交界处的薄壁细胞。在AR长出茎之前，AR中的维管组织发育并与茎的维管组织相连。 AR 中初级木质部极的数量约为 30 个。
There are about 4-6 slips on a fruit, and they are good materials for effective regeneration of Ananas comosus var. bracteatus. Adventitious root (AR) induction is essential for the propagation of Ananas comosus var. bracteatus slips. Growth regulator treatment, and culture medium are imperative factors that affect slip growth and rooting. In order to screen the optimal methods for slips rooting and reveal the anatomic procedure of slip rooting, this study induced slip rooting by different treatment of growth regulator, culture medium, observed the slip stem structure, AR origination and formation procedure through paraffin sections. The results showed that, slip cuttings treated with 100 mg/L of Aminobenzotriazole (ABT) for 6 hrs, cultured in river sand: coconut chaff: garden soil 2:2:1 medium is the optimal method for rooting. The proper supplementary of ABT can enhance the soluble sugar content, soluble protein content, polyphenol oxidase (PPO) activity and peroxidase (POD) enzyme activity, which resulted in the improvement of rooting. The slip stem structure is quite different from other monocots, which consists of epidermis, cortex, and stele with vascular tissues distributed in the cortex and stele. The AR primordia originates from the parenchyma cells located on the borderline between the cortex and stele. The vascular tissues in the AR develop and are connected with vascular tissue of the stem before the AR grew out the stem. The number of primary xylem poles in AR is about 30.