Synthesis, localization and possible function of serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in mouose submandibular gland.
Synthesis, localization and possible function of serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in mouose submandibular gland.
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小鼠颌下腺中丝氨酸（或半胱氨酸）肽酶抑制剂、进化枝 B、成员 6a (Serpinb6a) 的合成、定位和可能功能。
DOI：
10.1007/s00441-017-2620-1
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发表时间：
2017
影响因子：
3.6
通讯作者：
Iseki S
中科院分区：
文献类型：
--
作者：
Nakata H;Yamamoto M;Kumchantuek T;Adhapanywanich K;Nishiuchi T;Iseki S
下颌下腺导管系统中的颗粒曲小管（GCT）在雄性小鼠中优先发育，并产生许多生物活性肽，包括蛋白酶如肾素和激肽释放酶。我们研究的丝氨酸（或半胱氨酸）肽酶抑制剂，进化枝B，成员6a（Serpinb 6a），小鼠直系同源的人细胞内丝氨酸蛋白酶抑制剂SERPINB 6，在小鼠SMG的合成和本地化，通过使用逆转录加聚合酶链反应，原位杂交，免疫印迹和免疫组织化学。Serpinb 6a mRNA表达在男性比女性SMG和GCT比其他导管部分或腺泡中更丰富。在GCT细胞内，Serpinb 6a的免疫反应定位于细胞核和胞质溶胶中，但在分泌颗粒中不存在。通过使用免疫沉淀产物的质谱分析研究了SMG中Serpinb 6a的结合靶点，并鉴定了激肽释放酶-1相关肽酶b26（Klk 1b 26），一种丝氨酸蛋白酶。这些结果提高了Serpinb 6a在保护GCT细胞免受可能从分泌颗粒泄漏的细胞内激肽释放酶影响中起作用的可能性。
The granular convoluted tubule (GCT) in the duct system of the submandibular gland (SMG) develops preferentially in male mice and produces a number of bioactive peptides including proteases such as renin and kallikrein. We examine the synthesis and localization of the serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a), the mouse ortholog of the human intracellular serine protease inhibitor SERPINB6, in the mouse SMG by using reverse transcription plus the polymerase chain reaction, in situ hybridization, immunoblotting and immunohistochemistry. Serpinb6a mRNA expression was more abundant in the male than in the female SMG and in the GCT than in other duct portions or acini. Within GCT cells, immunoreactivity for Serpinb6a was localized in the nucleus and cytosol but was absent in the secretory granules. The binding target of Serpinb6a in the SMG was investigated by using a mass spectrometric analysis of immunoprecipitation products and kallikrein-1-related peptidase b26 (Klk1b26), a serine protease, was identified. These results raise the possibility that Serpinb6a functions in the protection of GCT cells from intracellular kallikreins that may leak from secretory granules.