CLONING OF HUMAN GENES ENCODING NOVEL G-PROTEIN-COUPLED RECEPTORS
CLONING OF HUMAN GENES ENCODING NOVEL G-PROTEIN-COUPLED RECEPTORS
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DOI：
10.1006/geno.1994.1549
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发表时间：
1994-10-01
期刊：
影响因子：
4.4
通讯作者：
ODOWD, BF
中科院分区：
文献类型：
--
作者：
MARCHESE, A;DOCHERTY, JM;ODOWD, BF
我们报告了几个新的人类基因编码G蛋白偶联受体的分离和鉴定。每个受体都含有熟悉的七个跨膜拓扑结构和最相似的肽结合受体。GPR 1基因编码一种受体蛋白，其编码区无内含子，与阿片受体具有相同性（跨膜区43%）。北方印迹分析显示，GPR 1转录本在人海马中表达，并且该基因定位于染色体15 q21.6。基因GPR 2编码一种与白细胞介素-8受体最相似的蛋白质（51%在跨膜区），该基因在检查的六个大脑区域中不表达，定位于染色体17q21.1-q21.3。第三个基因，GPR 3，显示出同一性（56%的跨膜区）与先前表征的大鼠cDNA克隆，并定位于染色体1 p35-p36.1。(C)1994年出版社出版。
We report the isolation and characterization of several novel human genes encoding G protein-coupled receptors. Each of the receptors contained the familiar seven transmembrane topography and most closely resembled peptide binding receptors. Gene GPR1 encoded a receptor protein that is intronless in the coding region and that shared identity (43% in the transmembrane regions) with the opioid receptors. Northern blot analysis revealed that GPR1 transcripts were expressed in the human hippocampus, and the gene was localized to chromosome 15q21.6. Gene GPR2 encoded a protein that most closely resembled an interleukin-8 receptor (51% in the transmembrane regions), and this gene, not expressed in the six brain regions examined, was localized to chromosome 17q21.1-q21.3. A third gene, GPR3, showed identity (56% in the transmembrane regions) with a previously characterized cDNA clone from rat and was localized to chromosome 1p35-p36.1. (C) 1994 Academic Press,Inc.