Expression of cell adhesion molecules in murine placentas and a placental cell line
Expression of cell adhesion molecules in murine placentas and a placental cell line
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DOI：
10.1095/biolreprod60.2.428
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发表时间：
1999-02-01
影响因子：
3.6
通讯作者：
Hunt, JS
中科院分区：
文献类型：
--
作者：
Bowen, JA;Hunt, JS
整合素和血管细胞粘附分子-1（VCAM-1）是胎盘正常发育所必需的。在这项研究中，研究了整合素亚基α 4、α v、β 1和β 3以及VCAM-1在子宫胎盘单位（妊娠日[g.d.] 6和8）和胎盘（g. d. 10、12、14、16和18）。所有亚基和VCAM-1 mRNA（通过逆转录聚合酶链反应[RT-PCR]鉴定）和蛋白（通过免疫荧光检测）在整个妊娠期的所有组织中存在。VCAM-1在胎盘外锥和滋养层巨细胞中强烈表达，α 4在滋养层巨细胞中强烈表达，在海绵滋养层和胎盘滋养层中中度表达，α v在海绵滋养层中的表达比在胎盘带中更强烈。β 1在胎盘中的表达比在海绵滋养层中更强，而β 3和VCAM-1在两个区域中基本相等。RT-PCR和免疫细胞化学检测结果显示，滋养层样SM 9 -1细胞对所有粘附分子均呈阳性反应。粘附分子在SM 9 -1细胞中的表达与谷胱甘肽区中的表达一致。总的来说，本研究的结果表明，鼠胎盘含有α 4、α v、β 1、β 3和VCAM-1的mRNA和蛋白质，并且表达是细胞特异性的。这些结果和鉴定的粘附分子表达的滋养层细胞系，应有利于未来的研究粘附分子在胎盘发育中的功能。
Integrins and vascular cell adhesion molecule-1 (VCAM-1) are required for normal placental development. In this study, integrin subunits alpha 4, alpha v, beta 1, and beta 3, and VCAM-1 were investigated for expression in uteroplacental units (gestation day [g.d.] 6 and 8) and placentas (g.d. 10, 12, 14, 16, and 18) of Swiss-Webster mice. All subunits and VCAM-1 mRNA (identified by reverse transcriptase polymerase chain reaction [RT-PCR]) and protein (detected by immunofluorescence) were present in all tissues throughout gestation. VCAM-1 was expressed strongly in the ectoplacental cone and trophoblast giant cells, alpha 4 was expressed strongly by trophoblast giant cells and moderately by spongiotrophoblast and labyrinthine trophoblast, and alpha v was expressed more strongly in the spongiotrophoblast than in the labyrinthine zone. The beta 1 was more strongly expressed in the labyrinthine than the spongiotrophoblast zone, while beta 3 and VCAM-1 were essentially equal in the two zones. Trophoblast-like SM9-1 cells were positive for all of the adhesion molecules when tested by RT-PCR and immunocytochemistry. Adhesion molecule expression in SM9-1 cells was consistent with expression in the labyrinthine zone. Collectively, the results of this study demonstrate that murine placentas contain mRNA and protein for alpha 4, alpha v, beta 1, beta 3, and VCAM-1, and that expression is cell-specific. These results and the identification of an adhesion molecule-expressing trophoblastic cell line should facilitate future studies on the function of adhesion molecules in placental development.