Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice.
Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice.
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DOI：
10.1139/g96-004
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发表时间：
1996-02
期刊：
影响因子：
3.1
通讯作者：
Naweed I. Naqvi;Bharat B. Chattoo
中科院分区：
文献类型：
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作者：
Naweed I. Naqvi;Bharat B. Chattoo
鉴定出两个随机扩增的多态性 DNA (RAPD) 标记 OPF6(2700) 和 OPH18(2400)，它们与 Pi-10 紧密连锁，Pi-10 是一种显性抗稻瘟病基因，赋予对稻瘟菌分离株 106（国际小种 IB46）的完全抗性。为了从 OPF6(2700) 和 OPH18(2400) 中获得序列特征扩增区域 (SCAR)，对这些扩增的 RAPD 产物进行了克隆和测序。使用从两个连接的 RAPD 标记的每一端获得的核苷酸序列信息来设计用于各自 SCAR 的 PCR 扩增的 24 聚体寡核苷酸引物。 Pi-10 的间接选择考虑了表现为 SCAR 替代等位基因长度差异的多态性。这种多态性将连锁的显性 RAPD 位点转化为共显性 SCAR 标记，并且还促进了抗稻瘟病和稻瘟病敏感基因型的间接评分。描述了与水稻稻瘟病抗性主要基因连锁的长度变异共显性 SCAR 标记的开发。共显性 SCAR 将促进水稻育种计划中 Pi-10 基因座的标记辅助选择，也可用作 Pi-10 区域高分辨率绘图的遗传标记。
Two randomly amplified polymorphic DNA (RAPD) markers, OPF6(2700) and OPH18(2400), tightly linked to Pi-10, a dominant blast-resistance gene conferring complete resistance to isolate 106 (international race IB46) of the blast fungus were identified. To derive sequence characterized amplified regions (SCARs) from OPF6(2700) and OPH18(2400), these amplified RAPD products were cloned and sequenced. Nucleotide sequence information, obtained for each end of the two linked RAPD markers, was used to design 24-mer oligonucleotide primers for PCR amplification of the respective SCARs. Polymorphisms appearing as differences in the length of the SCAR's alternate alleles were considered for the indirect selection of Pi-10. Such polymorphisms converted the linked dominant RAPD loci into codominant SCAR markers and also facilitated the indirect scoring of the blast-resistant and blast-susceptible genotypes. The development of length variant codominant SCAR markers linked to a major gene for blast resistance in rice is described. The codominant SCARs will facilitate marker-assisted selection of the Pi-10 locus in rice breeding programs and will also be useful as genetic markers for high resolution mapping of the Pi-10 region.