Wingless and Hedgehog signaling pathways regulate orthodenticle and eyes absent during ocelli development in Drosophila
Wingless and Hedgehog signaling pathways regulate orthodenticle and eyes absent during ocelli development in Drosophila
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DOI：
10.1016/j.ydbio.2009.02.027
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发表时间：
2009-05-01
影响因子：
2.7
通讯作者：
Gehring, Walter J.
中科院分区：
文献类型：
--
作者：
Blanco, Jorge;Seimiya, Makiko;Gehring, Walter J.
同源异型盒基因orthodenticle（old）控制的过程中发生在果蝇眼触角盘ocelli发展的区域规范。减少或消除单眼原基中旧表达的突变产生无单眼果蝇。我们已经确定了顺式调控序列（ocelliless增强子），控制旧的表达在单眼发育，并研究了其在分子水平上的调节。无眼增强子最初通过无翅（Wg）和刺猬（Hh）信号传导途径的联合作用被激活。随后，一个积极的自我调节反馈回路开始维持otd的表达。此外，我们分析了年龄在单眼原基发育过程中的作用，并确定其参与视网膜决定基因眼缺失（eya）的表达。old通过抑制wg（一种Eya阻遏物）和维持眼球原基中HH的表达来间接调节眼球前体细胞中的Eya。Hh信号是单眼前体细胞中Eya激活所必需的，并且这种激活由转录因子Cubitus interruptus的全长激活剂形式介导。(C)2009 Elsevier Inc. All rights reserved.
The homeobox gene orthodenticle (old) controls the process of regional specification that takes place in the Drosophila eye-antennal disc during ocelli development. Mutations that reduce or abolish old expression in the ocelli primordium give rise to ocelliless flies. We have identified the cis-regulatory sequence (ocelliless enhancer) that controls old expression during ocelli development and studied its regulation at the molecular level. The ocelliless enhancer is initially activated by the combined action of Wingless (Wg) and Hedgehog (Hh) signaling pathways. Later, a positive autoregulatory feedback loop sets in to maintain otd expression. Moreover, we have analyzed the role of old during ocelli primordium development and determined its involvement in the expression of the retinal determination gene eyes absent (eya). old indirectly regulates eya in ocellar precursor cells through the inhibition of wg, an eya repressor, and the maintenance of hh expression in the ocelli primordium. Hh signaling is necessary for eya activation in ocellar precursor cells and this activation is mediated by the full-length activator form of the transcription factor Cubitus interruptus. (C) 2009 Elsevier Inc. All rights reserved.