A CYCLIN-A-PROTEIN KINASE COMPLEX POSSESSES SEQUENCE-SPECIFIC DNA-BINDING ACTIVITY - P33CDK2 IS A COMPONENT OF THE E2F-CYCLIN-A COMPLEX
A CYCLIN-A-PROTEIN KINASE COMPLEX POSSESSES SEQUENCE-SPECIFIC DNA-BINDING ACTIVITY - P33CDK2 IS A COMPONENT OF THE E2F-CYCLIN-A COMPLEX
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DOI：
10.1016/0092-8674(92)90215-x
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发表时间：
1992-01-10
期刊：
影响因子：
64.5
通讯作者：
NEVINS, JR
中科院分区：
文献类型：
--
作者：
DEVOTO, SH;MUDRYJ, M;NEVINS, JR
已发现E2F转录因子与细胞周期蛋白A相关，该复合体在细胞周期的S时相积累，提示E2F可能在细胞周期调控中发挥作用。在独立研究中，细胞周期蛋白A已被证明与另外两种蛋白有关，即与Rb相关的p107蛋白和与cdc2相关的p33CDK2蛋白激酶。通过对E2F-Cyclin A复合体的分析，我们现在发现p107蛋白和cdc2相关的p33cdk2激酶都是前面描述的复合体的组成部分。此外，该复合体还具有H1激酶活性。因此，这些结果定义了具有序列特异性DNA结合活性的细胞周期蛋白A-CDK2激酶复合体。这表明CDK2激酶可以磷酸化其他DNA结合的底物，E2F因子的一个作用可能是将该蛋白激酶定位到DNA上。
The E2F transcription factor has been found in association with the cyclin A protein, and this complex accumulates during the S phase of the cell cycle, suggesting that E2F may play a role in cell cycle control. In independent studies, cyclin A has been shown to be associated with two other proteins, the Rb-related p107 protein and the cdc2-related p33 cdk2 protein kinase. Through an analysis of the E2F-cyclin A complex, we now find that both the p107 protein and the cdc2-related p33cdk2 kinase are components of the previously described complex. Moreover, the complex possesses H1 kinase activity. These results thus define a cyclin A-cdk2 kinase complex that possesses sequence-specific DNA binding activity. This suggests that the cdk2 kinase may phosphorylate other DNA-bound substrates, and that one role of the E2F factor may be to localize this protein kinase to the DNA.