Anti-MBL autoantibodies in patients with rheumatoid arthritis: prevalence and clinical significance
Anti-MBL autoantibodies in patients with rheumatoid arthritis: prevalence and clinical significance
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DOI：
10.1016/j.jaut.2006.07.002
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发表时间：
2006-09-01
影响因子：
12.8
通讯作者：
Das, Hasi Rani
中科院分区：
文献类型：
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作者：
Gupta, Bhawna;Raghav, Sunil Kumar;Das, Hasi Rani
患者血清中出现自身抗体是自身免疫性疾病的特征。我们通过酶免疫测定法评估了 107 名类风湿性关节炎 (RA) 患者和 121 名对照受试者血清中抗甘露糖结合凝集素 (MBL) 自身抗体的存在情况。首次检测到 RA 患者血清中抗 MBL 自身抗体水平升高（P < 0.0001）。 RA患者和对照组抗MBL阳性率分别为60.7%和1.65%。然后设计实验来了解这些自身抗体的功能相关性。观察到抗 MBL 自身抗体与血清 MBL 水平 (P = 0.001) 和 MBL 复合物活性 (P = 0.02) 呈负相关，但 MBL 多态性和抗 MBL 自身抗体分泌之间没有遗传关联。与血清样本相比，在 23 份滑液样本中观察到抗 MBL 自身抗体水平显着增加 (P = 0.038)。而且。与类风湿因子（RF）的 IgM 和 IgG 同种型相比，抗 MBL 自身抗体更常见于 RA 患者血清中（敏感性为 60.75%，特异性为 98.35%，ROC 曲线下面积为 0.913），当两种 RF 同种型均为阴性时，25.23% RA 患者的抗 MBL 自身抗体仍呈阳性。此外，在 RA 患者中，在疾病活动和关节畸形的所有阶段，抗 MBL 自身抗体比两种 RF 同种型更常见。因此，抗MBL自身抗体的显着存在表明抗MBL自身抗体可能具有诊断价值，然而，需要更多的研究来证实抗MBL自身抗体在类风湿关节炎诊断中的作用。 (c) 2006 Elsevier Ltd. 保留所有权利。
Occurrence of autoantibodies in patients' sera is the characteristic feature of autoimmune disorders. We assessed the presence of anti-mannose binding lectin (MBL) autoantibodies in the sera of 107 rheumatoid arthritis (RA) patients and 121 control subjects by enzyme immunoassay. Elevated levels of anti-MBL autoantibodies in the sera of RA patients (P < 0.0001) was detected for the first time. The ratios of anti-MBL positive in RA patients and controls were respectively 60.7% and 1.65%. Experiments were then designed to understand the functional relevance of these autoantibodies. An inverse correlation of anti-MBL autoantibodies with serum MBL levels (P = 0.001) and MBL complex activity (P = 0.02) was observed without genetic association between MBL polymorphisms and anti-MBL autoantibody secretion. A significant increase (P = 0.038) in the level of anti-MBL autoantibodies was observed in 23 synovial fluid samples in comparison to the serum samples. Moreover. the anti-MBL autoantibodies were found to be more often present in the sera of RA patients (60.75% sensitivity, 98.35% specificity and 0.913 area under the ROC curve) in comparison to the IgM and IgG isotypes of rheumatoid factors (RF), Anti-MBL autoantibodies were still positive in 25.23% RA patients when both the RF isotypes were negative. Also, in RA patients, at all stages of disease activity and joint deformity, anti-MBL autoantibodies were more often present than both the RF isotypes. Therefore, the significant presence of anti-MBL autoantibodies enunciates that anti-MBL autoantibodies might have a diagnostic valued however, more studies are needed to confirm the role of anti-MBL autoantibodies in the diagnosis of rheumatoid arthritis. (c) 2006 Elsevier Ltd. All rights reserved.