Changes in phycospheric and environmental microbes associated with an outbreak of yellow spot disease on Pyropia yezoensis
Changes in phycospheric and environmental microbes associated with an outbreak of yellow spot disease on Pyropia yezoensis
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与条斑紫菜黄斑病爆发相关的藻圈和环境微生物的变化
DOI：
10.1016/j.aquaculture.2020.735651
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发表时间：
2020-12-15
期刊：
影响因子：
4.5
通讯作者：
Yang, Rui
中科院分区：
文献类型：
--
作者：
Liu, Qiqin;Zhi, Yajun;Yang, Rui
藻圈微生物的组成和多样性对藻类的健康至关重要。条斑Pyropia yezoensis是一种在亚洲广受欢迎的食用海藻，黄斑病是条斑Pyropia yezoensis丝状体孢子培养期间的一种严重病害。研究了条斑紫菜丝状体藻圈及周围海水中细菌植物群的变化。研究了四种类型的藻类条件：1）健康（之前没有YSD）; 2）YSD; 3）用ClO 2处理的YSD和4）恢复（处理后14天）。在YSD样品中，微生物多样性显著降低。已报道的具有溶藻和降解功能的细菌（Kangiella、Alteromonas、Kordiimona、Tenacibaculum和Winogradskyella）的丰度显著增加，而一些有益细菌（Ruegeria和Bdellovibrio）的数量显著减少（P <0.05）。两组细菌之间呈负相关（P <0.05）。经ClO 2处理后，回收样品的藻类微生物群落恢复到正常水平。ClO 2处理增加了潜在病原菌（Loktanella和Kangiella）的丰度，降低了有益菌（Ruegeria和Bdellovibrio）的丰度（P <0.05）。化学处理对藻类微生物群落的影响是破坏性的和长期的。因此，应谨慎使用化学品，并建议采用破坏性较小的方法，如生物治疗，用于藻类生产系统的疾病预防和控制。
The composition and diversity of phycospheric microbes are critical to the health of algae. Pyropia yezoensis is a popular edible seaweed in Asia and yellow spot disease (YSD) is a serious disease during its conchocelis sporeling culture period. We studied the changes of the bacterial flora in the phycosphere and the surrounding seawater of P. yezoensis conchocelis during a YSD outbreak and treatment. Four types of algal conditions were studied 1) healthy (no previous YSD); 2) YSD; 3) YSD being treated with ClO2 and 4) recovery (14 d after treatment). In the YSD samples, the microbial diversity was significantly decreased. The abundance of bacteria that were reported having algal dissolving and degrading functions (Kangiella, Alteromonas, Kordiimona, Tenacibaculum and Winogradskyella) was significantly increased, and some beneficial bacteria (Ruegeria and Bdellovibrio) had significantly decreased populations (P < .05). There were negative correlations between these two groups of bacteria (P < .05). After treatment with ClO2, the algal microbial diversities of the recovery samples were restored to normal levels. However, ClO2 treatment increased the abundance of some potential pathogens (Loktanella and Kangiella), and decreased the abundance of beneficial bacteria such as Ruegeria and Bdellovibrio (P < .05). The effects of chemical treatment on the algal microbial community were disruptive and prolonged. Therefore, chemicals should be used cautiously, and less disruptive methods such as biological therapy are recommended for disease prevention and control in algal production systems.