ISOLATION AND EXPRESSION OF CDNA FOR DIFFERENT FORMS OF HEPATOCYTE GROWTH-FACTOR FROM HUMAN-LEUKOCYTE
ISOLATION AND EXPRESSION OF CDNA FOR DIFFERENT FORMS OF HEPATOCYTE GROWTH-FACTOR FROM HUMAN-LEUKOCYTE
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DOI：
10.1016/s0006-291x(05)80212-8
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发表时间：
1990-10-15
影响因子：
3.1
通讯作者：
SHIMIZU, S
中科院分区：
文献类型：
--
作者：
SEKI, T;IHARA, I;SHIMIZU, S
以人肝脏cDNA为探针，筛选人白细胞cDNA文库，分离编码肝细胞生长因子的cDNA克隆。对获得的4个克隆中的2个进行了核苷酸和推断的氨基酸序列分析。其中一个包含一个开放阅读框，编码一个728个氨基酸残基的多肽链，类似于来自人肝脏的cDNA克隆。在另一个克隆中，在编码序列中发现了15个碱基对的自发缺失。在体外用COS-1细胞短暂表达时，两个克隆产生的蛋白对大鼠肝细胞具有相似的生物活性。
Human leukocyte cDNA library was screened to isolate cDNA clones coding for hepatocyte growth factor using cDNA from human liver as a probe. Nucleotide and deduced amino acid sequences were analyzed for two of four clones obtained. One of them contained an open reading frame coding for a polypeptide chain of 728 amino acid residues like that of cDNA clone derived from human liver. In another clone a spontaneous deletion of 15 base pairs was found within the coding sequence. When expressed transiently using COS-1 cells both clones produced protein with similar biological activity against rat hepatocyte in vitro.