The Behavior of Lux-marked Ralstonia, solanacearum in Grafted Tomato Cultivars Resistant or Susceptible to Bacterial Wilt
The Behavior of Lux-marked Ralstonia, solanacearum in Grafted Tomato Cultivars Resistant or Susceptible to Bacterial Wilt
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勒克斯标记的青枯菌在抗青枯病嫁接番茄品种中的表现
DOI：
10.3186/jjphytopath.65.597
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
T. Okuno
中科院分区：
文献类型：
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作者：
Y. Hikichi;Y. Nakazawa;Shinobu Kitanosono;Kazumi Suzuki;T. Okuno
用带有一个弧菌的luxCDABE操纵子和一个来自于Burkholderia glumae基因组DNA的启动子区的pNP 126转化青枯雷尔氏菌OE 1 -1。由此获得的来自YN 5的生物发光强度与细菌在体外从停滞期到稳定期的生长正相关。在这项研究中，我们使用的Vim相机配备了ARGUS 50连续观察生物发光和青枯病的发展，在单，双嫁接番茄植株的各种组合的抗性LS-89和敏感Oogata福寿。细菌在番茄根和颈中的生长程度是引起番茄青枯病的决定因素之一。LS-89的抗性是由于抑制了第一片叶以下的根和茎中细菌的增殖。在使用LS-89作为砧木的嫁接番茄植株中，所有砧木均潜伏感染细菌。即使植物没有枯萎，细菌也从敏感的接穗中回收。在某些植物中，细菌在感病的接穗中增殖。这些植物枯萎得很厉害。总之，抑制抗性砧木根和第一叶以下茎中的细菌增殖影响了感病接穗中细菌的生长和对病害发展的抗性的定量控制。
Ralstonia solanacearum OE1-1 was transformed with pNP126 carrying a luxCDABE operon of Vibrio fisheri and a promoter region derived from the genomic DNA of Burkholderia glumae. Intensity of bioluminescence from YN5 thus obtained positively correlated with growth of the bacteria from the lag phase to the stationary phase in vitro. In this study, we used the VIM camera equipped with the ARGUS 50 to successively observe bioluminescence and the development of bacterial wilt in singly and doubly grafted tomato plants in various combinations of the resistant LS-89 and the susceptible Oogata-Fukuju. The degree of bacterial growth in the roots and collars was one of the determinants for the induction of bacterial wilt in tomato plants. Resistance of LS-89 resulted from suppression of bacterial proliferation in the roots and stems below the first leaf. In grafted tomato plants using LS-89 as the rootstock, all rootstocks were latently infected with the bacteria. The bacteria were also recovered from susceptible scions even though the plants did not wilt. In some plants, the bacteria proliferated in the susceptible scions. These plants wilted heavily. Taken together, suppression of bacterial proliferation in the roots and the stems below the first leaf of resistant rootstocks affected the bacterial growth in the susceptible scions and quantative control of resistance against development of the disease.