Clinicopathological significance of microRNA‐21 in extracellular vesicles of pleural lavage fluid of lung adenocarcinoma and its functions inducing the mesothelial to mesenchymal transition
Clinicopathological significance of microRNA‐21 in extracellular vesicles of pleural lavage fluid of lung adenocarcinoma and its functions inducing the mesothelial to mesenchymal transition
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DOI：
10.1002/cam4.2928
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发表时间：
2020-02
期刊：
影响因子：
4
通讯作者：
Shiori Watabe;Y. Kikuchi;S. Morita;D. Komura;S. Numakura;Arisa Kumagai-Togashi;Masato Watanabe
中科院分区：
文献类型：
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作者：
Shiori Watabe;Y. Kikuchi;S. Morita;D. Komura;S. Numakura;Arisa Kumagai-Togashi;Masato Watanabe
术前胸腔灌洗细胞学检查有助于预测肺癌患者的肿瘤复发和预后。近年来，从渗出液标本中分离的细胞外囊泡（EV）受到关注，一些研究表明EV中的microRNA与预后相关。MicroRNA-21（miR-21）是一种代表性的肿瘤microRNA，EV中的miR-21（EV-miR-21）通过诱导腹膜腔中的间皮细胞向间质细胞转化（MMT）促进癌症扩散。在这项研究中，我们从胸膜灌洗液中分离EV，并将EV-miR-21作为与胸膜传播相关的诊断因素。
Pre‐resection pleural lavage cytology is useful to predict tumor recurrence and the prognosis of lung cancer patients. Recently, extracellular vesicles (EVs) isolated from effusion specimens have come under the spotlight, and several studies showed that microRNA in EVs is associated with prognosis. MicroRNA‐21 (miR‐21) is a representative onco‐microRNA, and miR‐21 in EVs (EV‐miR‐21) promotes cancer dissemination by inducing mesothelial to mesenchymal transition (MMT) in the peritoneal cavity. In this study, we isolated EVs from pleural lavage fluid and focused on EV‐miR‐21 as a diagnostic factor with a relationship to pleural dissemination.