Effect of matrix stiffness on the proliferation and differentiation of umbilical cord mesenchymal stem cells
Effect of matrix stiffness on the proliferation and differentiation of umbilical cord mesenchymal stem cells
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基质硬度对脐带间充质干细胞增殖和分化的影响
DOI：
10.1016/j.diff.2017.07.001
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发表时间：
2017-07-01
期刊：
影响因子：
2.9
通讯作者：
Li, Yulin
中科院分区：
文献类型：
--
作者：
Xu, Juanjuan;Sun, Meiyu;Li, Yulin
间充质干细胞（MSC）是一种兼容的细胞替代再生医学和组织工程，因为他们强大的多能性。基质硬度对干细胞行为起着深远的作用。然而，基质硬度对脐带间充质干细胞（UC-MSCs）的影响尚未研究。为了进行深入的探索，我们将UC-MSC培养在不同刚度（杨氏模量：13-16、35-38、48-53和62-68 kPa）的涂有纤连蛋白的聚丙烯酰胺凝胶上。结果发现，UC-MSCs在不同基质上的增殖和黏附能力不同，随着刚度的增加，UC-MSCs的铺展能力增强（*P < 0.05）。实时定量PCR结果显示，软基质促进成脂分化，具有较高的脂肪细胞标志物如PPAR的表达水平。C/EBPa（*P < 0.05）。相反，当在48-53 kPa基质上培养时，细胞倾向于分化成肌肉，这通过肌原性标记物如结蛋白和MOYG的表达增加来验证（*P < 0.05）。此外，成骨细胞标记物（*P < 0.05），如ALP、I型胶原、骨钙素和Runx 2的表达增加，证实了细胞在高刚度基质上分化成骨。
Mesenchymal stem cells (MSCs) are a compatible cellular alternative for regenerative medicine and tissue engineering because of their powerful multipotency. Matrix stiffness plays a profound role on stem cell behavior. Nevertheless, the effect of matrix stiffness on umbilical cordmesenchymal stem cells (UC-MSCs) remains unexplored. To conduct an in-depth exploration, we cultured UC-MSCs on different stiffness (Young's modulus: 13-16, 35-38, 48-53, and 62-68 kPa) polyacrylamide gels coated with fibronectin. We found that the proliferation and adhesion of UC-MSCs varied when cultured on the different matrices, and the spreading capacity was stronger as the stiffness increased (*P < 0.05). Real-time quantitative PCR results showed that the soft matrix promoted adipogenic differentiation, with higher expression levels of adipocytic markers like PPAR. and C/EBPa (*P < 0.05). In contrast, cells tended to differentiate into muscle when cultured on the 48-53 kPa matrix, which was validated by increased expression of myogenic makers like desminand MOYG (*P < 0.05). Moreover, increased expression of osteoblastic makers (*P < 0.05), such as ALP, collagen type I, osteocalcin, and Runx2, confirmed that cells differentiated into bone on the high-stiffness matrix.