Extra-hepatic vitamin A concentrations in captive Rhesus (Macaca mulatta) and Marmoset (Callithrix jacchus) monkeys fed excess vitamin A.
Extra-hepatic vitamin A concentrations in captive Rhesus (Macaca mulatta) and Marmoset (Callithrix jacchus) monkeys fed excess vitamin A.
复制标题
饲喂过量维生素 A 的圈养恒河猴 (Macaca mulatta) 和狨猴 (Callithrix jacchus) 的肝外维生素 A 浓度。
DOI：
10.1024/0300-9831.75.2.126
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Tanumihardjo,SherryA
中科院分区：
文献类型：
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作者：
Mills,JordanP;Penniston,KristinaL;Tanumihardjo,SherryA
最近对用作人类生物医学研究模型的恒河猴 (Macaca mulatta) 的维生素 A (VA) 状况进行了检查，结果发现肝脏 VA 浓度处于低毒性。另一种实验动物狨猴 (Callithrix jacchus) 的肝脏中 VA 含量也很高，血清视黄酯浓度也很高。这两个物种都食用普通的研究饮食，所提供的 VA（乙酸视黄酯）含量是国家研究委员会目前建议的四倍。为了进一步明确许多人类亚群中长期高膳食VA的影响，我们分析了低毒性恒河猴和狨猴（每只n = 10）的肺和肾组织中的视黄醇和视黄酯。狨猴肾脏含有 0.88 ± 0.66 mumol VA/g，与肝 VA 几乎相同，为 1.40 ± 0.44 mumol/g (p = 0.143)。相比之下，恒河猴肾 VA 浓度为 0.0100 ± 0.0032 mumol/g，尽管肝脏储备为 18.8 ± 6.4 mumol VA/g (p < 0.0001)。狨猴和恒河猴的肺组织 VA 浓度分别为 0.0022 ± 0.0012 和 0.0061 ± 0.0025 mumol/g，低于肾脏 (p < 0.011)。猴子的肾脏和肺部 VA 储备充足但不过量，尚未确定；因此，对这些发现的解释仅限于组织视黄醇和视黄酯概况以及从其他物种的推断，而不是与“正常”值直接比较。
Recent work examining vitamin A (VA) status of rhesus monkeys (Macaca mulatta) used as models for human biomedical research has revealed subtoxic hepatic VA concentrations. Livers of marmoset monkeys (Callithrix jacchus), another experimental animal, were also high in VA as was serum retinyl ester concentration. Both species consumed common research diets that provided up to four times the amount of VA (retinyl acetate) as currently recommended by the National Research Council. To further define the effects of chronically high dietary VA as found in many human subpopulations, we analyzed lung and kidney tissues from subtoxic rhesus and marmoset monkeys (n = 10 each) for retinol and retinyl esters. Marmoset kidneys contained 0.88 ± 0.66 mumol VA/g and was nearly the same as hepatic VA at 1.40 ± 0.44 mumol/g (p = 0.143). In contrast, rhesus kidney VA concentrations were 0.0100 ± 0.0032 mumol/g, even though liver reserves were 18.8 ± 6.4 mumol VA/g (p < 0.0001). Lung tissue VA concentrations, 0.0022 ± 0.0012 and 0.0061 ± 0.0025 mumol/g for marmosets and rhesus, respectively, were lower as compared with kidney (p < 0.011). Kidney and lung VA in monkeys with adequate, but not excessive, VA stores have not been determined; hence, interpretation of these findings is limited to tissue retinol and retinyl ester profiles and extrapolation from other species rather than direct comparison to "normal" values.