Abnormal nuclear expression of Pygopus-2 in human primary hepatocellular carcinoma correlates with a poor prognosis
Abnormal nuclear expression of Pygopus-2 in human primary hepatocellular carcinoma correlates with a poor prognosis
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Pygopus-2 在人原发性肝细胞癌中的异常核表达与不良预后相关。
DOI：
10.1111/his.12637
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发表时间：
2015-08-01
期刊：
影响因子：
6.4
通讯作者：
Wang, Xiao-Min
中科院分区：
文献类型：
--
作者：
Zhang, Sheng;Li, Jie;Wang, Xiao-Min
目的Pygopus-2（Pygo 2）是经典Wnt/β-catenin转录复合物的关键元件。本研究旨在探讨Pygo 2在原发性肝细胞癌（HCC）中的表达模式及其临床病理意义。方法采用实时荧光定量聚合酶链反应（PCR）技术检测50例HCC癌组织和癌旁正常肝组织中Pygo 2 mRNA的表达水平。免疫组化分析显示，Pygo 2蛋白在HCC患者中的异常表达与年龄（P=0.025）、肿瘤大小（P=0.005）、肝内或肝外转移（P=0.029）、血管侵犯（P=0.026）和肿瘤分化程度（P=0.004）有关。Pygo 2蛋白表达正常的患者生存时间较长（P=0.031），1年生存率较高（P=0.032）。考克斯的比例风险回归模型显示Pygo 2蛋白的异常表达是影响肝癌患者预后的危险因素（P=0.043）。我们的研究结果表明，Pygo 2可能是HCC患者预后不良的重要预测因子，并可作为HCC的新生物标志物。
AimsPygopus-2 (Pygo2) is a critical element of the canonical Wnt/-catenin transcriptional complex. The aim of the present study was to investigate the expression patterns and clinicopathological significance of Pygo2 in human primary hepatocellular carcinoma (HCC).Methods and resultsReal-time polymerase chain reaction (PCR) analysis of the mRNA levels of Pygo2 in 50 paired HCC cancer/adjacent non-cancerous tissues showed that Pygo2 mRNA expression was significantly higher in cancerous tissues (P=0.009). Immunohistochemical analysis showed that abnormal Pygo2 protein expression in HCC patients was associated with age (P=0.025), tumour size (P=0.005), intra- or extra-hepatic metastasis (P=0.029), vascular invasion (P=0.026) and tumour differentiation (P=0.004). Patients with normal Pygo2 protein expression showed a longer survival time (P=0.031) and a higher 1-year survival rate (P=0.032) than those with abnormal Pygo2 expression. Cox's proportional hazard regression model showed that abnormal Pygo2 protein expression was a risk factor associated with the prognosis of HCC patients (P=0.043).ConclusionTo the best of our knowledge, this is the first report investigating Pygo2 expression patterns and their clinicopathological significance in HCC. Our findings suggest that Pygo2 may be an important predictor of poor outcome in HCC patients, and could serve as a novel biomarker for HCC.