INDEPENDENT TRANSFER OF MITOCHONDRIAL CHROMOSOMES AND PLASMIDS DURING UNSTABLE VEGETATIVE FUSION IN NEUROSPORA
INDEPENDENT TRANSFER OF MITOCHONDRIAL CHROMOSOMES AND PLASMIDS DURING UNSTABLE VEGETATIVE FUSION IN NEUROSPORA
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DOI：
10.1038/345177a0
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发表时间：
1990-05-10
期刊：
影响因子：
64.8
通讯作者：
SAVILLE, BJ
中科院分区：
文献类型：
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作者：
COLLINS, RA;SAVILLE, BJ
类似内含子在细胞器、核糖体 RNA 和噬菌体基因中的零星分布表明，这些内含子中至少有一些是可移动的遗传元件1-3。真菌线粒体中的一些质粒含有类似内含子的序列4，并且像内含子一样，它们在物种内部和物种之间分散分布5-7。这种 DNA 水平转移的发生和进化重要性，不仅在真菌之间，而且在广泛的生物体之间，一直是人们广泛讨论的问题1-3,8-12。在这里，我们报告了在不稳定的营养细胞融合过程中神经孢子线粒体质粒从一种线粒体基因型高频转移到另一种线粒体基因型的实验证据。线粒体和核基因组的交换也可能发生。这些观察结果表明，营养融合在自然群体的线粒体遗传结构中可能比通常认为的更重要，并且可能为某些质粒和可能的其他可移动遗传元件的分布提供解释。
THE sporadic distribution of similar introns in organelle, nuclear ribosomal RNA and bacteriophage genes suggests that at least some of these introns are mobile genetic elements1–3. Some plasmids in fungal mitochondria contain intron-like sequences4and, like introns, they have scattered distributions within and among species5–7. The occurrence and evolutionary importance of such horizontal transfer of DNA, not only between fungi, but among a wide range of organisms have been matters of much discussion1–3,8–12. Here, we report experimental evidence for transfer ofNeurosporamitochondrial plasmids from one mitochondrial genotype to another at high frequency during unstable vegetative cell fusion. Exchange of mitochondrial and nuclear genomes can also occur. These observations suggest that vegetative fusion may have a more important role in the mitochondrial genetic structure of natural populations than is generally thought, and may provide an explanation for the distribution of certain plasmids and possibly other mobile genetic elements.