Structure Analysis and Anti-Tumor and Anti-Angiogenic Activities of Sulfated Galactofucan Extracted from Sargassum thunbergii
Structure Analysis and Anti-Tumor and Anti-Angiogenic Activities of Sulfated Galactofucan Extracted from Sargassum thunbergii
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DOI：
10.3390/md17010052
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发表时间：
2019-01-01
期刊：
影响因子：
5.4
通讯作者：
Zhong, Weihong
中科院分区：
文献类型：
--
作者：
Jin, Weihua;Wu, Wanli;Zhong, Weihong
硫酸化岩藻半乳聚糖（ST-2）是从鼠尾藻中分离得到的。再经水解得到ST-2-DS，再经乙醇自水解沉淀得到上清液（ST-2-S）和沉淀（ST-2-C）。ST-2-C通过凝胶色谱进一步分级分离成两个级分，ST-2-H（高分子量）和ST-2-L（低分子量）。对ST-2-DS进行质谱分析（MS），以阐明ST-2的骨架。结果表明，ST-2-DS的骨架结构是由吡喃半乳糖（Gal）（n）（n3）和吡喃岩藻糖（Fuc）（n）交替组成.此外，还通过MS确定了ST-2-S，以阐明ST-2的分支。这表明，硫酸化岩藻糖低聚物可能是ST-2的分支。与ST-2-H的NMR谱相比，ST-2-L的NMR谱更易识别。结果表明，ST-2-L含有（Gal）（n）和（Fuc）（n）的主链，主要在Fuc的C4处硫酸化，并散布有半乳糖（键可能是12和16）。因此，ST-2可能含有（Gal）（n）（n 3）和（Fuc）（n）的主链。硫酸化模式主要发生在吡喃岩藻糖的C4位，部分发生在吡喃半乳糖的C4位，支链主要为硫酸化岩藻糖低聚物。最后，测定了ST-2及其衍生物的抗肿瘤和抗血管生成活性。结果表明，岩藻糖含量较高的低分子量硫酸化岩藻半乳聚糖具有较好的抗血管生成和抗肿瘤活性。
Sulfated galactofucan (ST-2) was obtained from Sargassum thunbergii. It was then desulfated to obtain ST-2-DS, and autohydrolyzed and precipitated by ethanol to obtain the supernatant (ST-2-S) and precipitate (ST-2-C). ST-2-C was further fractionated by gel chromatography into two fractions, ST-2-H (high molecular weight) and ST-2-L (low molecular weight). Mass spectrometry (MS) of ST-2-DS was performed to elucidate the backbone of ST-2. It was shown that ST-2-DS contained a backbone of alternating galactopyranose residues (Gal)(n) (n 3) and fucopyranose residues (Fuc)(n). In addition, ST-2-S was also determined by MS to elucidate the branches of ST-2. It was suggested that sulfated fuco-oligomers might be the branches of ST-2. Compared to the NMR spectra of ST-2-H, the spectra of ST-2-L was more recognizable. It was shown that ST-2-L contain a backbone of (Gal)(n) and (Fuc)(n), sulfated mainly at C4 of Fuc, and interspersed with galactose (the linkages were likely to be 12 and 16). Therefore, ST-2 might contain a backbone of (Gal)(n) (n 3) and (Fuc)(n). The sulfation pattern was mainly at C4 of fucopyranose and partially at C4 of galactopyranose, and the branches were mainly sulfated fuco-oligomers. Finally, the anti-tumor and anti-angiogenic activities of ST-2 and its derivates were determined. It was shown that the low molecular-weight sulfated galactofucan, with higher fucose content, had better anti-angiogenic and anti-tumor activities.