Sry and Sox9 expression during canine gonadal sex determination assayed by quantitative reverse transcription-polymerase chain reaction.
Sry and Sox9 expression during canine gonadal sex determination assayed by quantitative reverse transcription-polymerase chain reaction.
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通过定量逆转录聚合酶链式反应测定犬性腺性别过程中 Sry 和 Sox9 的表达。
DOI：
10.1002/mrd.10317
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Meyers-Wallen,VN
中科院分区：
文献类型：
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作者：
Meyers-Wallen,VN
在哺乳动物中，睾丸诱导与性腺Sryand Sox9的表达有关，而在Sryis缺失的脊椎动物中，睾丸诱导与Sox9的表达有关。在哺乳动物中，Sry可能通过上调Sox9的表达来启动睾丸诱导；然而，缺乏直接证据支持这一假说。Sry阴性XX性反转(XXSR)模型，即睾丸在缺乏Sry的情况下发育，可以在睾丸诱导中提供Sry和Sox9之间的联系。采用定量逆转录聚合酶链式反应(qRT-PCR)检测SryandSox9在正常泌尿生殖道脊(UGR)和性腺组织中的表达，以明确犬睾丸发育的不同阶段。Sox9在雄性和雌性犬的UGR中均有表达，直到CS17，此时睾丸的表达是卵巢的10倍。虽然qRT-PCR检测到Sox9在雌雄两性的卵巢和中肾中均有表达，但整体原位杂交(WMISH)只在犬的睾丸中检测到Sox9的表达。Sry9和Sox9在睾丸中的表达时间与其在睾丸决定中的作用是一致的：Sry16在睾丸中开始表达，随后在CS17表达上调。在正常犬性腺中Sry9和Sox9表达的数量和时空模式与人、羊和猪相似。这些研究为了解Sry阴性XXSR犬模型的睾丸诱导机制奠定了基础。摩尔。是Reprod。戴夫。65：373-381,2003。©2003 Wiley-Liss公司
Testis induction is associated with gonadalSryandSox9expression in mammals, and withSox9expression in vertebrates whereSryis absent. In mammals,Srymight initiate testis induction by upregulatingSox9expression; however, direct evidence supporting this hypothesis is lacking. Models ofSry‐negative XX sex reversal (XXSR), in which testes develop in the absence ofSry, could provide the link betweenSryandSox9in testis induction. To define the stages at which testis determination occurs in the canine model,SryandSox9expression were measured in normal urogenital ridges (UGR) and gonads by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). TesticularSryexpression rose continuously during canine developmental ages comparable to human carnegie stages (CS) 16–18, with maximal expression at CS 18.Sox9was expressed in both male and female canine UGR up to CS 17, at which time testis expression became tenfold greater than in the ovary. AlthoughSox9was detected by qRT‐PCR in ovaries and mesonephroi of both sexes, expression was detected only in canine testes by whole mount in situ hybridization (WMISH). The timing ofSryandSox9expression is consistent with a role in testis determination:Sryexpression begins at CS 16 in testes, followed by upregulation ofSox9expression at CS 17. The quantity and temporal and spatial patterns ofSryandSox9expression in normal canine gonads are similar to those in humans, sheep, and pigs. These studies should provide the basis for understanding the mechanism of testis induction in the canine model ofSry‐negative XXSR. Mol. Reprod. Dev. 65: 373–381, 2003. © 2003 Wiley‐Liss, Inc.