INTEGRATION OF DNA FRAGMENTS BY ILLEGITIMATE RECOMBINATION IN SACCHAROMYCES-CEREVISIAE
INTEGRATION OF DNA FRAGMENTS BY ILLEGITIMATE RECOMBINATION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.88.17.7585
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
PETES, TD
中科院分区：
文献类型：
--
作者：
SCHIESTL, RH;PETES, TD
将BamHI处理产生的与酵母基因组没有同源性的DNA片段转化到酿酒酵母中。当这些片段在BamHI酶存在的情况下转化时，它们整合到基因组BamHI位点上。当这些片段在没有酶的情况下转化时，它们整合到基因组的G-A-T-C位点。由于G-A-T-C序列存在于BamHI片段的末端，这一结果表明，四个碱基对的同源性足以进行某些类型的有丝分裂重组。
DNA fragments (generated by BamHI treatment) with no homology to the yeast genome were transformed into Saccharomyces cerevisiae. When the fragments were transformed in the presence of the BamHI enzyme, they integrated into genomic BamHI sites. When the fragments were transformed in the absence of the enzyme, they integrated into genomic G-A-T-C sites. Since the G-A-T-C sequence is present at the ends of BamHI fragments, this result indicates that four base pairs of homology are sufficient for some types of mitotic recombination.