Effects of enriched endogenous omega-3 fatty acids on age-related hearing loss in mice
Effects of enriched endogenous omega-3 fatty acids on age-related hearing loss in mice
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DOI：
10.1186/s13104-019-4809-8
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发表时间：
2019-11-26
影响因子：
1.8
通讯作者：
Osumi, Noriko
中科院分区：
文献类型：
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作者：
Honkura, Yohei;Suzuki, Jun;Osumi, Noriko
目的饮食干预是预防老年性听力损失（阿勒）的有效措施。Omega-3（n-3）多不饱和脂肪酸（PUFAs）可能有效预防阿勒，因为它们具有抗炎和组织保护功能。研究了来自C57 BL/6 N品系的野生型和Fat-1转基因小鼠的听力功能与年龄相关的变化，这些小鼠可以将欧米茄-6 PUFA转化为n-3 PUFA，从而产生富集的内源性n-3 PUFA，以测试n-3 PUFA的功效3 PUFA预防阿勒。结果在2个月时，Fat-1和野生型小鼠在8-16 kHz下的基线听性脑干反应（ABR）阈值相同，但在4和32 kHz下Fat-1小鼠明显较高。相比之下，Fat-1小鼠的ABR阈值在10个月时显著降低。此外，在13个月（12 kHz）时，Fat-1小鼠在中低频率下的ABR阈值显著降低。在13个月时，Fat-1小鼠的体重显著降低，但在2、10和16-17个月时没有。总之，由于Fat-1转基因的表达而产生的富集的内源性n-3 PUFA部分减轻了雄性C57 BL/6 N小鼠中的阿勒。
Objective Dietary intervention is a practical prevention strategy for age-related hearing loss (AHL). Omega-3 (n-3) polyunsaturated fatty acids (PUFAs) may be effective in prevention of AHL due to their anti-inflammatory and tissue-protective functions. Age-related changes in the hearing function of wild-type and Fat-1 transgenic mice derived from the C57BL/6N strain, which can convert omega-6 PUFAs to n-3 PUFAs and consequently produce enriched endogenous n-3 PUFAs, were investigated to test the efficacy of n-3 PUFAs for AHL prevention. Results At 2 months, the baseline auditory brainstem response (ABR) thresholds were the same in Fat-1 and wild-type mice at 8-16 kHz but were significantly higher in Fat-1 mice at 4 and 32 kHz. In contrast, the ABR thresholds of Fat-1 mice were significantly lower at 10 months. Moreover, the ABR thresholds of Fat-1 mice at low-middle frequencies were significantly lower at 13 months (12 kHz). Body weights were significantly reduced in Fat-1 mice at 13 months, but not at 2, 10, and 16-17 months. In conclusion, enriched endogenous n-3 PUFAs produced due to the expression of the Fat-1 transgene partially alleviated AHL in male C57BL/6N mice.