Effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells
Effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells
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DOI：
10.3748/wjg.v12.i21.3359
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发表时间：
2006-06-07
影响因子：
4.3
通讯作者：
Yu-Bin Ji
中科院分区：
文献类型：
--
作者：
Shi-Yong Gao;Qiu-Juan Wang;Yu-Bin Ji
目的：目的：观察龙葵碱对HepG（2）细胞线粒体膜电位及细胞内[Ca 2 +] i的影响，探讨龙葵碱诱导HepG（2）细胞凋亡的机制。用TMRE染色HepG（2）细胞，用LCSM观察细胞线粒体膜电位的变化。用Fluo-3/AM双染法检测HepG（2）细胞内[Ca ~（2+）]（i）的变化。用TMRE和Fluo-3/AM双染HepG（2）细胞，LCSM观察细胞线粒体膜电位和细胞内[Ca ~（2+）] i的变化。TMRE染色显示龙葵碱可降低线粒体膜电位，Fluo-3/AM染色显示龙葵碱可增加肿瘤细胞内Ca 2+浓度，TMRE和Fluo-3/AM双重染色显示龙葵碱在降低线粒体膜电位的同时可增加细胞内Ca 2+浓度。龙葵碱通过降低膜电位打开膜中的PT通道，导致Ca 2+沿其浓度梯度向下转运，这反过来又导致细胞中Ca 2+浓度升高，开启细胞凋亡机制。(C)2006年，WJG出版社。All rights reserved.
AIM: To observe the effect of solanine on the membrane potential of mitochondria in HepG(2) cells and [Ca2+], in the cells, and to uncover the mechanism by which solanine induces apoptosis.METHODS: HepG(2) cells were double stained with AO/EB, and morphological changes of the cells were observed using laser confocal scanning microscopy (LCSM). HepG(2) cells were stained with TMRE, and change in the membrane potential of mitochondria in the cells were observed using LCSM. HepG(2) cells were double stained with Fluo-3/AM, and change of [Ca2+](i) in the cells were observed using LCSM. HepG(2) cells were double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+](i) in the cells were observed using LCSM.RESULTS: Cells in treated groups showed typical signs of apoptosis. Staining with TMRE showed that solanine could lower membrane potential; staining with Fluo-3/AM showed that solanine could increase the concentration of Ca2+ in tumor cells; and those of double staining with TMRE and Fluo-3/AM showed that solanine could increase the concentration of Ca2+ in the cells at the same time as it lowered the membrane potential of mitochondria.CONCLUSION: Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca2+ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca2+ in the cell, turning on the mechanism for apoptosis. (C) 2006 The WJG Press. All rights reserved.