Hydrogen sulfide alleviates cadmium-induced morpho-physiological and ultrastructural changes in Brassica napus
Hydrogen sulfide alleviates cadmium-induced morpho-physiological and ultrastructural changes in Brassica napus
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DOI：
10.1016/j.ecoenv.2014.08.027
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发表时间：
2014-12-01
影响因子：
6.8
通讯作者：
Zhou, Weijun
中科院分区：
文献类型：
--
作者：
Ali, Basharat;Gill, Rafaqat A.;Zhou, Weijun
以油菜（Brassica napus L.）为材料，研究了硫化氢（H2S）对镉（Cd）胁迫的缓解作用。在温室条件下进行了研究。水培植物生长在三个水平（0，100和500亩M）的镉和三个水平（0，100和200亩M）的H2S供体，硫氢化钠（NaHS）。结果表明，H2S处理显著促进了B的生长、根系形态、叶绿素含量、元素吸收和光合活性。镉胁迫下的油菜植株。此外，H2S处理降低了B叶片和根系中Cd的含量。镉胁迫下的油菜植株。外源H2S通过提高Cd胁迫下叶片和根系的抗氧化酶活性，降低了叶片和根系中丙二醛和活性氧的产生。显微镜观察表明，外源H_2S处理改善了叶肉细胞的结构，使叶肉细胞干净，叶绿体发达，类囊体膜完整，线粒体数量增多。H_2S和Cd联合处理下，根尖细胞线粒体、长内质网和高尔基体等结构发生变化。由此可见，外源H2S对B的生长、光合参数、元素吸收、抗氧化酶活性和超微结构变化具有保护作用。在高镉胁迫条件下，(C)2014爱思唯尔公司All rights reserved.
In the present study, role of hydrogen sulfide (H2S) in alleviating cadmium (Cd) induced stress in oilseed rape (Brassica napus L.) was studied under greenhouse conditions. Plants were grown hydroponically under three levels (0,100, and 500 mu M) of Cd and three levels (0,100 and 200 mu M) of H2S donor, sodium hydrosulfide (NaHS). Results showed that application of H2S significantly improved the plant growth, root morphology, chlorophyll contents, elements uptake and photosynthetic activity in B. napus plants under Cd stress. Moreover, addition of H2S reduced the Cd concentration in the leaves and roots of B. napus plants under Cd-toxicity. Exogenously applied H2S decreased the production of malondialdehyde and reactive oxygen species in the leaves and roots by improving the enzymatic antioxidant activities under Cd stress conditions. The microscopic examination indicated that application of exogenous H2S improved the cell structures and enabled a clean mesophyll cell having a well developed chloroplast with thylakoid membranes, and a number of mitochondria could be observed in the micrographs. A number of modifications could be found in root tip cell i.e. mature mitochondria, long endoplasmic reticulum and golgibodies under combined application of H2S and Cd. On the basis of these findings, it can be concluded that application of exogenous H2S has a protective role on plant growth, photosynthetic parameters, elements uptake, antioxidants enzyme activities and ultrastructural changes in B. napus under high Cd stress conditions. (C) 2014 Elsevier Inc. All rights reserved.