Overexpression of forkhead box J2 can decrease the migration of breast cancer cells
Overexpression of forkhead box J2 can decrease the migration of breast cancer cells
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叉头盒J2过度表达可减少乳腺癌细胞的迁移
DOI：
10.1002/jcb.24146
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发表时间：
2012-08-01
影响因子：
4
通讯作者：
Cheng, Chun
中科院分区：
文献类型：
--
作者：
Wang, Yingying;Yang, Shuyun;Cheng, Chun
乳腺癌转移患者的预后普遍较差，因此有必要阐明相关的分子机制。叉头盒J2(Forkhead Box J2，FOXJ2)是叉头盒转录因子中的一员，已有报道称其中许多转录因子参与了肿瘤的迁移和侵袭。在本研究中，FOXJ2在无淋巴结转移的乳腺癌组织中的表达高于有淋巴结转移的乳腺癌组织，且FXOJ2的表达与临床因素有统计学意义。因此，我们确定了一个新的转移功能，这是以前没有人知道的FOXJ2。创伤愈合实验和跨孔迁移实验显示，FOXJ2过表达降低了高迁移的MDA-MB-231细胞的运动能力，Western印迹分析、逆转录聚合酶链式反应(RT-PCR)和免疫荧光分析表明，FOXJ2过表达降低了细胞上皮标志物E-钙粘附素的表达和间质标志物Vimentin的表达。与这些观察结果一致的是，FOXJ2在弱转移的MCF-7细胞中的抑制显著促进了细胞的运动。我们的研究表明FOXJ2可以通过调节EMT关键标志物E-钙粘蛋白和波形蛋白来抑制人乳腺癌的转移。J.细胞。生物化学。2012年，113：2729-2737。©2012 Wiley期刊，Inc.
The prognosis of breast cancer patients with metastases is generally poor, so it is essential to elucidate related molecules mechanisms. Forkhead Box J2 (FOXJ2) is a member of Forkhead Box transcription factors, many of which have been reported to participate in tumor migration and invasion. In this study, we showed the expression of FOXJ2 was higher in primary breast cancer tissues without lymph nodes metastases than those with, and there was statistical significance between the expression of FXOJ2 and the clinical factors. Hence, we identified a novel function of metastasis, which was not previously known for FOXJ2. Overexpression of FOXJ2 decreased the motility property of highly migrative MDA‐MB‐231 cells in vitro by wound healing assays and trans‐well migration assays, and it was concurrent with the increased expression of epithelial marker E‐cadherin and the decreased expression of mesenchymal marker vimentin by Western blot analysis, reverse transcription PCR analysis, and immunofluorescence analysis. Consistent with these observations, the repression of FOXJ2 in weakly metastatic MCF‐7 cells remarkably promoted cellular motility. Our study demonstrates that FOXJ2 can inhibit the metastasis of human breast cancer by regulating the EMT key markers E‐cadherin and vimentin. J. Cell. Biochem. 113: 2729–2737, 2012. © 2012 Wiley Periodicals, Inc.