IDENTIFICATION OF VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI IN ROOTS OF LEEK (ALLIUM-PORRUM L) AND MAIZE (ZEA-MAYS-L) ON THE BASIS OF ENZYME MOBILITY DURING POLYACRYLAMIDE-GEL ELECTROPHORESIS
IDENTIFICATION OF VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI IN ROOTS OF LEEK (ALLIUM-PORRUM L) AND MAIZE (ZEA-MAYS-L) ON THE BASIS OF ENZYME MOBILITY DURING POLYACRYLAMIDE-GEL ELECTROPHORESIS
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DOI：
10.1111/j.1469-8137.1986.tb00829.x
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发表时间：
1986-04-01
期刊：
影响因子：
9.4
通讯作者：
MASKALL, CS
中科院分区：
文献类型：
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作者：
HEPPER, CM;SEN, R;MASKALL, CS
聚丙烯酰胺凝胶电泳中酯酶（EST）、谷草转氨酶（GOT）和肽酶（PEP）的迁移率可用于鉴定根系中的囊泡-丛枝菌根真菌。从菌根根提取物获得的酶带型进行了比较与未感染的根。厚垣孢子、外部菌丝体和内部菌丝体（通过宿主根组织的酶消化获得）用作对照以确认凝胶上真菌特异性酶带的位置。Glomus caledonium（Nicol.（Gerd）特拉普和格尔德和Glomus mosseae（Nicol.（Gerd）GERD.韭菜（Allium porrum L.）通过EST、GOT和PEP酶带的迁移率，可以检测到G. Caledonium和G.通过对这三种酶中的任何一种进行染色，在混合接种物存在下生长的韭菜根中的mosseae。在玉米（Zea mays L.）根中的EST、GOT和PEP活性的主要酶带，而根中的EST、GOT和PEP活性的主要酶带则主要在根中。caledonium具有与玉米相同的迁移性，因此使用这些实验条件在该宿主中不能容易地鉴定该真菌。球囊霉Glomus fasciculatum（Thaxter sensu Gerd.）GERD. TrappeE 3型的PEP谱带与韭菜和玉米寄主的PEP谱带相分离，并且该菌在玉米根中的GOT酶活性也可通过凝胶电泳的位置进行鉴定。
The mobilities of three enzymes, esterase (EST), glutamate oxaloacetate transaminase (GOT) and peptidase (PEP), during polyacrylamide gel electrophoresis have been used to identify individual species of vesicular-arbuscular mycorrhizal fungi in roots. The enzyme banding patterns obtained from mycorrhizal root extracts were compared with those of uninfected roots. Chlamydospores, external mycelium and internal mycelium (obtained by enzymic digestion of host root tissue) were used as controls to confirm the position of the fungal specific enzyme bands on the gels. Glomus caledonium (Nicol. and Gerd.) Trappe and Gerd. and Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe could be detected in leek (Allium porrum L.) roots against the host background by the mobility of bands of EST, GOT and PEP activity and it was possible to detect both G. caledonium and G. mosseae in leek roots which had been grown in the presence of a mixed inoculum, by staining for any of these three enzymes. Glomus mosseae could be identified in maize (Zea mays L.) roots by location of GOT and PEP activity but the major bands of EST, GOT and PEP activity in G. caledonium had the same mobility as those of maize and so this fungus could not be easily identified in this host using these experimental conditions. Glomus fasciculatum (Thaxter sensu Gerd.) Gerd. and Trappe type E3 had a characteristic PEP band which was separable from leek and maize host bands and this fungus could also be identified in maize roots by the position on the gel of fungal GOT activity.