A MIGRATING ELECTRIC COMPLEX OF CANINE SMALL INTESTINE
A MIGRATING ELECTRIC COMPLEX OF CANINE SMALL INTESTINE
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DOI：
10.1152/ajplegacy.1969.217.6.1757
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发表时间：
1969-01-01
影响因子：
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通讯作者：
SZURSZEWSKI, JH
中科院分区：
文献类型：
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作者：
SZURSZEWSKI, JH
材料和方法使用七只健康的雌性杂种狗（lo-1 2 kg）。在三只狗中，电位由 15 个单极和 5 个双极铂电极检测，在四只狗中，电位由 11 个单极电极检测。电极的结构和用于将它们连接到记录器的连接器是按照 Allen、Poole 和 Code (I) 所描述的模式设计的。单极电极由一根从聚四氟乙烯圆盘突出 0.5 毫米的铂丝组成。双极电极由两根相距 5 毫米的铂丝组成，每根铂丝的尖端从圆盘突出 0.5 毫米。通过使用手术无菌技术（没有使用预谋），当狗处于戊巴比妥麻醉（30毫克/千克，静脉注射）时，电极以相等或几乎相等的距离植入，第一个放置在十二指肠上，最后一个放置在回肠末端。三只狗使用的五个双极电极始终位于最远端，并且其检测尖端与肠的长轴成直角。手术后十天，开始记录，并定期持续3-6个月。这些狗意识清醒且健康，并接受训练在录音期间安静地躺在吊带中。观察过程持续至少3.5小时，在此期间，使用1秒的时间常数在带有直线墨水书写系统的8通道记录仪（Brush Mark 200）上记录电势。在肠上的电极和放置在右大腿皮下的铂针电极之间获得单极电位。双极性电位是在肠上相隔 5 mm 的电极之间获得的。如果回肠中的慢波起源于十二指肠中的横向平面(2)，那么从理论上讲，双极记录应该无法记录慢波信号。然而，在实践中，获得了慢波，并且与来自同一区域的单极记录相比，记录更稳定并且包含更少的运动伪影。一种解释可能是双极电极的记录尖端不垂直于纵向肌纤维，因为当植入电极时，没有采取特殊的预防措施来确保它们完全垂直。大部分实验都完成了
MATERIALS AND METHODSSeven healthy, female, mongrel dogs (lo-1 2 kg) were used. In three dogs, the electric potentials were detected by 15 unipolar and 5 bipolar platinum electrodes, and in four dogs, they were detected by 11 unipolar electrodes. The construction of the electrodes and the connector used to unite them to a recorder was patterned after those described by Allen, Poole, and Code (I). The unipolar electrodes consisted of one platinum wire which protruded 0.5 mm from the Teflon disk. The bipolar electrodes consisted of two platinum wires separated by 5 mm, the tip of each protruding 0.5 mm from the disk. With the use of surgical aseptic technique(no premeditations were used) while the dog was under pentobarbital anesthesia (30 mg/kg, intravenously), the electrodes were implanted at equal or very nearly equal distances, the first being placed on the duodenum and the last on the terminal ileum. The five bipolar electrodes, used in three dogs, were always the most distal and were oriented so that their detecting tips were at right angles to the long axis of the bowel. Ten days after the operation, recording sessions were begun, and these were continued periodically for 3-6 months. The dogs were conscious and healthy and were trained to lie quietly in a sling during periods of recording. The observation sessions lasted for a minimum of 3.5 hr, during which the electric potentials were registered on an 8-channel recorder(Brush Mark 200) with a rectilinear inkwriting system using a time constant of l sec. Unipolar potentials were obtained between the electrodes on the bowel and a platinum needle electrode placed subcutaneously in the right thigh. Bipolar potentials were obtained between the electrodes on the bowel separated by 5 mm. If the slow wave in the ileum originated in the transverse plane as in the duodenum(2) then, theoretically, bipolar recording should cant el the slow-wave signal. In practice however, slow w ‘aves were obtained, and the recordings were more stable and contained fewer movement artifacts than the unipolar recordings from the same region. An explanation might be that the recording tips of the bipolar electrodes were not perpendicular to the longitudinal muscle fibers, for when the electrodes were implanted, no particular precautions were taken to ensure that they were exactly perpendicular. Most experiments were done