RECOVERY OF DENGUE VIRUSES FROM PATIENTS DURING EPIDEMICS IN PUERTO RICO AND EAST PAKISTAN
RECOVERY OF DENGUE VIRUSES FROM PATIENTS DURING EPIDEMICS IN PUERTO RICO AND EAST PAKISTAN
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DOI：
10.4269/ajtmh.1966.15.573
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发表时间：
1966-01-01
影响因子：
3.3
通讯作者：
ORDONEZ, J
中科院分区：
文献类型：
--
作者：
RUSSELL, PK;BUESCHER, EL;ORDONEZ, J
从波多黎各(1963年)和东巴基斯坦(1964年)暴发的临床登革热患者的急性期血液中发现了与登革3型病毒密切相关(如果不是相同的话)的病毒。由于每次流行的病毒在乳鼠或断奶小鼠接种后几乎不会引起任何明显的疾病，因此这些病毒的恢复和鉴定完全依赖于使用挑战病毒抗性测试的细胞培养技术。原代非洲绿猴肾(GMK)细胞或连续异倍体GMK细胞(BS-C-1)的单层培养对登革热病毒的检测比二倍体早期传代的BS-C-1细胞更敏感。对新回收的病毒在原细胞中进行定期和可重复的滴定。细胞培养中繁殖的登革热病毒的滴度太低，不能证明补体固定或血凝抗原，但足以进行中和试验，以识别新恢复的毒株。描述了最终在GMK细胞培养系统中恢复和鉴定登革热病毒的技术。
Viruses, closely related if not identical to dengue virus type 3, were recovered from the acute phase bloods of persons with clinical dengue fever in outbreaks in Puerto Rico (1963) and East Pakistan (1964). Because viruses from each epidemic induced little if any overt disease upon inoculation of suckling or weanling mice, the recovery and identification of these viruses were dependent entirely upon cell culture techniques using the challenge virus resistance test. Monolayer of cultures of primary African green monkey kidney (GMK) cells, or continuous heteroploid GMK cells (BS-C-1) were found to be more sensitive for detection of dengue viruses than was the diploid early passage BS-C-1 cell. Titrations ol newly recovered viruses were regularly and reproducibly made in the former cells. Titers of cell culture propagated dengue viruses were too low to permit demonstration of complement fixing or hemagglutinating antigen, but adequate for performance of neutralization tests to identify newly recovered strains. The techniques ultimately developed for recovery and characterization of dengue viruses in the GMK cell culture system are described.