Use of multi‐InDels as novel markers to analyze 13 X‐chromosome haplotype loci for forensic purposes
Use of multi‐InDels as novel markers to analyze 13 X‐chromosome haplotype loci for forensic purposes
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DOI：
10.1002/elps.201500159
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发表时间：
2015-12
期刊：
影响因子：
2.9
通讯作者：
Guangyao Fan;Y. Ye;Haibo Luo;Yiping Hou
中科院分区：
文献类型：
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作者：
Guangyao Fan;Y. Ye;Haibo Luo;Yiping Hou
许多研究已提出鉴定插入/删除（InDel）多态性在人类法医遗传学研究。然而，双等位基因标记的低多态性限制了InDels的鉴别能力。为了提高它们的区分能力，我们开发了multi - InDel，这是一种新的常染色体标记，由两个以上的InDel位点组成，这些InDel位点通过它们的物理位置紧密相连，并通过一对PCR引物组合成一个特定的标记。该策略为每个多InDel标记提供至少三个单倍型。这种标记在法医应用中可能非常有用。在这项研究中，我们重点研究了位于X染色体（ChrX）上的多InDel标记。开发了包含13个多InDel标记的多路系统，其中包括ChrX中的28个InDel位点。为了验证多InDel面板，研究了群体样本和一组家系中的单倍型分布。本研究证明了这些标记对个体识别和关系研究的有用性。我们强调，位于ChrX上的多InDel标记可以为复杂的亲属关系检测提供新的支持信息。
Many studies have been proposed to identify insertion/deletion (InDel) polymorphisms in humans for forensic genetic studies. However, the discriminatory power of InDels is limited by the poor polymorphisms of diallelic markers. To improve their discriminatory power, we developed multi‐InDel, a novel autosomal marker comprising more than two InDel loci that are tightly linked by their physical position and combined into a specific marker by a pair of PCR primers. This strategy gives at least three haplotypes for each multi‐InDel marker. Such markers can be potentially very useful in forensic applications. In this study, we focused on multi‐InDel markers located on X chromosome (ChrX). A multiplex system with 13 multi‐InDel markers, including 28 InDel loci in ChrX, was developed. To validate the multi‐InDel panel, the haplotype distribution in a population sample and in a set of pedigrees was investigated. This study demonstrates usefulness of these markers for individual identification and relationship studies. We highlight the fact that the multi‐InDel markers located on ChrX can provide new supporting information for complex kinship testing.