Maturation of nonadrenergic noncholinergic inhibitory system in normal and allergen-sensitized rabbits.
Maturation of nonadrenergic noncholinergic inhibitory system in normal and allergen-sensitized rabbits.
复制标题
正常和过敏原致敏兔子中非肾上腺素能非胆碱能抑制系统的成熟。
DOI：
10.1152/ajplung.1994.267.6.l739
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Larsen,GL
中科院分区：
文献类型：
--
作者：
Colasurdo,GN;Loader,JE;Graves,JP;Larsen,GL
我们研究了非肾上腺素能非胆碱能抑制（NANCi）系统在体外发育的兔气道中的功能存在。此外，我们评估了胃肠外暴露于特定过敏原（豚草）对该神经通路成熟的影响。在正常和豚草致敏的新西兰白色兔1、2、4和12周龄的气管平滑肌（TSM）段上研究了NANCi反应。取下TSM节段，置于含有改良Krebs-Henseleit溶液、阿托品（1 × 10 - 5 M）和普萘洛尔（5 × 10 - 6 M）的组织浴中。在用神经激肽A（1 × 10（-5）M）收缩后，以5至30 Hz的刺激频率施加电场刺激，以确定产生最大舒张的频率。测量NANCi对EFS的反应，并表示为20 Hz时的平均（+/- SE）松弛百分比，因为该刺激频率在每个研究年龄段均产生最大NANCi反应。在1周龄时从对照兔获得的TSM节段在所使用的刺激频率下未表现出NANCi反应。相比之下，一个可重复的NANC松弛证明在TSM从2，4，和12周龄的兔子。该反应的幅度分别为27 +/- 4.7（n = 10）、29 +/- 4.8（n = 9）和37 +/- 4%（n = 18）。对从豚草致敏动物获得的TSM片段进行的相同实验给出了显著降低的NANCi响应值。在2、4和12周龄家兔中，NANCi反应分别为11.5 +/- 3.4（n = 9）、11 +/- 2（n = 13）和16 +/- 4.2%（n = 14）。（250字处删节）
We investigated the functional existence of the nonadrenergic noncholinergic inhibitory (NANCi) system in developing rabbit airways in vitro. Furthermore, we evaluated the effect of parenteral exposure to a specific allergen (ragweed) on the maturation of this neural pathway. NANCi responses were studied on tracheal smooth muscle (TSM) segments obtained from normal and ragweed-sensitized New Zealand White rabbits at 1, 2, 4, and 12 wk of age. The TSM segments were removed and placed in tissue baths containing modified Krebs-Henseleit solution, atropine (1 x 10(-5) M), and propranolol (5 x 10(-6) M). After contraction with neurokinin A (1 x 10(-5) M), electrical field stimulation was applied at stimulation frequencies ranging from 5 to 30 Hz to determine the frequency that produced maximal relaxation. The NANCi response to EFS was measured and expressed as the mean (+/- SE) percent relaxation at 20 Hz, because this stimulation frequency gave the maximal NANCi response at each age studied. TSM segments obtained from control rabbits at 1 wk of age did not demonstrate a NANCi response at the frequencies of stimulation used. By contrast, a reproducible NANC relaxation was demonstrated in TSM from 2-, 4-, and 12-wk-old rabbits. The magnitude of this response was 27 +/- 4.7 (n = 10), 29 +/- 4.8 (n = 9), and 37 +/- 4% (n = 18), respectively. The same experiments performed on TSM segments obtained from ragweed-sensitized animals gave significantly decreased values of NANCi response. In 2-, 4-, and 12-wk-old rabbits, the NANCi responses were 11.5 +/- 3.4 (n = 9), 11 +/- 2 (n = 13), and 16 +/- 4.2% (n = 14).(ABSTRACT TRUNCATED AT 250 WORDS)