Papillary and follicular thyroid carcinomas show distinctly different microarray expression profiles and can be distinguished by a minimum of five genes
Papillary and follicular thyroid carcinomas show distinctly different microarray expression profiles and can be distinguished by a minimum of five genes
复制标题
DOI：
10.1200/jco.2004.08.127
复制
发表时间：
2004-09-01
影响因子：
45.3
通讯作者：
Eng, C
中科院分区：
文献类型：
--
作者：
Aldred, MA;Huang, Y;Eng, C
目的：我们以前对两种最常见的非髓样甲状腺癌，即甲状腺乳头状癌（PTC）和甲状腺滤泡状癌（FTC）进行了独立的微阵列表达分析。在这项研究中，我们试图联合收割机，我们的数据集，揭示这些肿瘤type.Materials和MethodsMicroarray数据之间的相似性和差异，从6个PTC，9个FTCs，和13个正常甲状腺样本进行了规范化，以消除实验室间的差异，然后分析无监督聚类，t检验，并通过比较的绝对和变化的电话。通过逆转录聚合酶链反应对这些相同的样本，再加上8个额外的FTC tumors.ResultsPTC显示两组不同的基因，要么过表达或低表达与正常甲状腺相比，而在FTCs的主要变化是表达下降，在以前没有牵连在甲状腺癌的基因的表达变化进行了验证。五个基因可以共同区分这两种肿瘤类型。PTCs显示CITED 1、claudin-10（CLDN10）和胰岛素样生长因子结合蛋白6（IGFBP6）的过表达，但未显示小窝蛋白-1（CAV 1）或小窝蛋白-2（CAV 2）的表达变化;相反，FTC不表达CLDN10，IGFBP6和/或CAV 1和CAV 2表达降低。结论PTC和FTC具有不同的表达谱，这表明它们要么具有不同的分子起源，要么它们从共同的起源明显地分化。此外，如果在更大范围的肿瘤中得到验证，这些基因可以与已知的肿瘤特异性染色体易位结合，形成有价值的诊断工具的基础。(C)2004年，美国临床肿瘤学会。
PurposeWe have previously conducted independent microarray expression analyses of the two most common types of nonmedullary thyroid carcinoma, namely papillary thyroid carcinoma (PTC) and follicular thyroid carcinoma (FTC). In this study, we sought to combine our data sets to shed light on the similarities and differences between these tumor types.Materials and MethodsMicroarray data from six PTCs, nine FTCs, and 13 normal thyroid samples were normalized to remove interlaboratory variability and then analyzed by unsupervised clustering, t test, and by comparison of absolute and change calls. Expression changes in four genes not previously implicated in thyroid carcinogeness were verified by reverse transcriptase polymerase chain reaction on these same samples, together with eight additional FTC tumors.ResultsPTCs showed two distinct groups of genes that were either over- or underexpressed compared with normal thyroid, whereas the predominant changes in FTCs were of decreased expression. Five genes could collectively distinguish the two tumor types. PTCs showed overexpression of CITED1, claudin-10 (CLDN10), and insulin-like growth factor binding protein 6 (IGFBP6) but showed no change in expression of caveolin-1 (CAV1) or -2 (CAV2); conversely, FTCs did not express CLDN10 and had decreased expression of IGFBP6 and/or CAV1 and CAV2.ConclusionPTC and FTC show distinctive microarray expression profiles, suggesting that either they have different molecular origins or they diverge distinctly from a common origin. Furthermore, if verified in a larger series of tumors, these genes could, in combination with known tumor-specific chromosome translocations, form the basis of a valuable diagnostic tool. (C) 2004 by American Society of Clinical Oncology.