Reproduction, larval growth, and reproductive development in African clawed frogs (Xenopus laevis) exposed to atrazine
Reproduction, larval growth, and reproductive development in African clawed frogs (Xenopus laevis) exposed to atrazine
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DOI：
10.1016/j.chemosphere.2007.09.051
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发表时间：
2008-03-01
期刊：
影响因子：
8.8
通讯作者：
Van Der Kraak, Glen J.
中科院分区：
文献类型：
--
作者：
Du Preez, Louis H.;Kunene, Nisile;Van Der Kraak, Glen J.
研究了暴露于阿特拉津的F1非洲爪蟾（Xenopus laevis）在整个幼虫发育和性成熟期的F2后代的繁殖成功率和发育情况。幼虫X从受精后96小时开始，并持续至变态后两年，将非洲乳鼠暴露于四种标称浓度的阿特拉津（0、1、10、25微克阿特拉津/升）中的一种。窝卵数和后代的存活率被用作衡量F1青蛙繁殖成功的测量终点。幼虫存活率和变态时间被用来衡量阿特拉津暴露青蛙的F2后代的发育成功。对F1和F2代青蛙的睾丸进行了检查，以确定异常的发生率，如睾丸卵泡，并对F2代后代的性别比例进行了调查，以确定阿特拉津暴露是否会引起跨代效应（由于F1个体暴露而对F2个体产生的效应）。阿特拉津的任何研究浓度对F1青蛙的窝卵数没有影响。对孵化成功率或变态时间也没有影响。处理组间F2子代的性别比无差异。没有证据表明阿特拉津对X的产卵成功率或生殖发育有跨代影响。光滑。这与X的强大种群的存在是一致的。几十年来，阿特拉津一直被用于玉米生产中的杂草控制。我们的观察结果也与大多数其他青蛙研究的结果一致，其中没有发现与阿特拉津接触有关的影响。我们的数据不支持阿特拉津显著影响青蛙生殖适合度和发育的假设。(c)2007爱思唯尔有限公司保留所有权利。
Reproductive success and development of F2 offspring from F1 adult African clawed frogs (Xenopus laevis) exposed to atrazine throughout larval development and as sexually mature adults was examined. Larval X. laevis were exposed to one of four nominal concentrations of atrazine (0, 1, 10, 25 mu g atrazine/l) beginning 96 hr after fertilization and continuing through two years post-metamorphosis. Clutch size and survival of offspring were used as measurement endpoints to gauge reproductive success of the F1 frogs. Larval survivorship and time to metamorphosis were used to gauge developmental success of the F2 offspring from atrazine-exposed frogs. Testes in F1 and F2 frogs were examined for incidence of anomalies, such as testicular ovarian follicles, and sex ratios in F2 offspring were investigated to determine if exposure to atrazine caused trans-generational effects (effects on F2 individuals due to exposure of F1 individuals). There were no effects of any of the studied concentrations of atrazine on clutch size of F1 frogs. There were also no effects on hatching success or time to metamorphosis. Sex ratios did not differ between F2 offspring among treatments. There was no evidence to suggest a transgenerational effect of atrazine on spawning success or reproductive development of X. laevis. This is consistent with the presence of robust populations of X. laevis in areas where they are exposed to atrazine that has been used for several decades for weed control in production of corn. Our observations also are consistent with the results of most other studies of frogs where no effects were found to be associated with exposure to atrazine. Our data do not support the hypothesis that atrazine significantly affects reproductive fitness and development of frogs. (c) 2007 Elsevier Ltd. All rights reserved.