Rhodococcus rhodochrous ATCC12674 becomes alkane-tolerant upon GroEL2 overexpression and survives in the n-octane phase in two phase culture.
Rhodococcus rhodochrous ATCC12674 becomes alkane-tolerant upon GroEL2 overexpression and survives in the n-octane phase in two phase culture.
复制标题
Rhodococcus Rhodochrous ATCC12674在Groel2过表达时变为烷烃耐受性，并且在两期培养中在N-辛烷阶段生存。
DOI：
10.1264/jsme2.me14114
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发表时间：
2014
影响因子：
2.2
通讯作者：
Sunairi M
中科院分区：
文献类型：
--
作者：
Takihara H;Matsuura C;Ogihara J;Iwabuchi N;Sunairi M
我们最近报道了GroEL2的过表达在增加红红红球菌PR4的烷烃耐受性中起重要作用。在本研究中，我们研究了引入groEL2对其他红球菌菌株烷烃耐受性的影响。将groEL2引入红球菌菌株可提高其对烷烃的耐受性。引入PR4衍生的groEL2可显著提高R. rhodochrous ATCC12674细胞在两相培养中向C8相的易位和存活，表明工程细胞过表达groEL2是提高红球菌有机溶剂耐耐性的有效策略。
We recently reported that the overexpression of GroEL2 played an important role in increasing the alkane tolerance of Rhodococcus erythropolis PR4. In the present study, we examined the effects of the introduction of groEL2 on the alkane tolerance of other Rhodococcus strains. The introduction of groEL2 into Rhodococcus strains led to increased alkane tolerance. The translocation of R. rhodochrous ATCC12674 cells to and survival in the n-octane (C8) phase in two phase culture were significantly enhanced by the introduction of groEL2 derived from strain PR4, suggesting that engineering cells to overexpress GroEL2 represents an effective strategy for enhancing organic solvent tolerance in Rhodococcus.