MUTATIONS AFFECTING THE PATTERN OF THE LARVAL CUTICLE IN DROSOPHILA-MELANOGASTER .2. ZYGOTIC LOCI ON THE 3RD CHROMOSOME
MUTATIONS AFFECTING THE PATTERN OF THE LARVAL CUTICLE IN DROSOPHILA-MELANOGASTER .2. ZYGOTIC LOCI ON THE 3RD CHROMOSOME
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DOI：
10.1007/bf00848157
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
KLUDING, H
中科院分区：
文献类型：
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作者：
JURGENS, G;WIESCHAUS, E;KLUDING, H
本报告描述了黑腹果蝇第三染色体合子位点突变等位基因的恢复和遗传特征，这些突变等位基因改变了幼虫角质层的形态。我们从甲烷磺酸乙酯（EMS）处理的核核染色体中获得12600个单系，并通过估算卵子的孵化率来检测它们的胚胎致死突变。其中约有7100个系产生了至少四分之一的未孵化卵，然后对胚胎表型进行评分。对未孵化的卵进行显微检查，将1772个株系归为胚胎致死株系，占全部致死株系的24%。在198个株系（占全部致死株系的2.7%）中，突变胚胎表现出明显的幼虫表皮异常。这些胚胎可见突变体通过互补分析确定了45个位点。在32个位点中，发现了一个以上的突变等位基因，平均每个位点有5.8个等位基因。13个突变体与其他所有突变体互补。通过重组分析在遗传图谱上定位，并通过缺陷互补分析在细胞学上定位。它们似乎是沿着染色体随机分布的。等位基因频率和与缺陷表型的比较表明，这45个位点代表了第三染色体上的大多数（如果不是全部的话）合子位点，其中功能缺失明显影响幼虫角质层的形态。
The present report describes the recovery and genetic characterization of mutant alleles at zygotic loci on the third chromosome ofDrosophila melanogasterwhich alter the morphology of the larval cuticle. We derived 12600 single lines from ethyl methane sulfonate (EMS)-treatedst eorrucucachromosomes and assayed them for embryonic lethal mutations by estimating hatch rates of egg collections. About 7100 of these lines yielded at least a quarter of unhatched eggs and were then scored for embryonic phenotypes. Through microscopic examination of unhatched eggs 1772 lines corresponding to 24% of all lethal hits were classified as embryonic lethal. In 198 lines (2.7% of all lethal hits), mutant embryos showed distinct abnormalities of the larval cuticle. These embryonic visible mutants define 45 loci by complementation analysis. For 32 loci, more than one mutant allele was recovered, with an average of 5.8 alleles per locus. Complementation of all other mutants was shown by 13 mutants. The genes were localized on the genetic map by recombination analysis, as well as cytologically by complementation analysis with deficiencies. They appear to be randomly distributed along the chromosome. Allele frequencies and comparisons with deficiency phenotypes indicate that the 45 loci represent most, if not all, zygotic loci on the third chromosome, where lack of function recognizably affects the morphology of the larval cuticle.