Designed structural-rearrangement of an active group I ribozyme
Designed structural-rearrangement of an active group I ribozyme
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DOI：
10.1093/jb/mvg023
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发表时间：
2003-02-01
影响因子：
2.7
通讯作者：
Inoue, T
中科院分区：
文献类型：
--
作者：
Ikawa, Y;Inoue, T
四膜虫I族核酶的催化核心由两个功能不同的结构域P4-P6和P3-P7组成，它们通过多个三级相互作用连接在一起。编码P3-P7结构域的序列在其主序列中被分成两个片段，尽管这两个结构域在核酶的三维(3D-)结构中是物理上可分离的：编码P4-P6结构域的序列被插入到P3-P7结构域的序列中。根据核酶的三维结构模型和生化数据，设计并尝试了P3-P7结构域一级序列的人工重排。一级结构中的结构域被重新定位以形成一个连续的区域，同时保留了自剪接所需的核酶的3D结构。拓扑重排的核酶具有自剪接活性。
The catalytic core of the Tetrahyemena group I ribozyme consists of two functionally different domains, P4-P6 and P3-P7, that are conjugated via multiple tertiary interactions. The sequence encoding the P3-P7 domain is divided into two fragments in its primary sequence although the two domains are physically separable in the three dimensional (3D-) structure of the ribozyme: The sequence encoding the P4-P6 domain is inserted into that of the P3-P7 domain. An artificial rearrangement was designed and attempted for the primary sequence of the P3-P7 domain on the basis of a 3D-structural model and the biochemical data on the ribozyme. The domain in the primary structure was relocated to form a contiguous region while retaining the 3D-structure of the ribozyme required for self-splicing. The topologically rearranged ribozyme exhibited self-splicing activity.