Construction of a linkage map and QTL analysis for black rot resistance in Brassica oleracea L.
Construction of a linkage map and QTL analysis for black rot resistance in Brassica oleracea L.
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DOI：
10.3329/ijns.v1i1.8591
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Okazaki, K.
中科院分区：
文献类型：
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作者：
Doullah, M. A. U.;Mohsin, G. M.;Okazaki, K.
对控制油菜黄单胞菌致病变种抗性的数量性状基因座（QTL）进行了分析。本研究以感病青花菜和抗病甘蓝[绿色Commet P09 * Reiho P01]的F3家系为材料，利用酶切扩增多态性序列（CAPS）和序列相关扩增多态性（SRAP）分析构建了连锁图谱。我们建立了接种技术。在该技术中，通过在靠近边缘的中脉处切割1.0cm来接种来自约50天龄F3植物的叶。共筛选了38对CAPS和60对SRAP引物，以评估亲本对黑腐病抗性的多态性。92个标记分布在10个连锁群中，标记间距平均为3.56cM，标记总长度为320.5cM。LG 2和LG 9上的两个基因组区域与抗病性显著相关。在LG 2上的CAM 1-GSA 1之间的图区间上发现了一个QTL，解释了10%的表型变异;在LG 9上的F12-R12 e-BORED之间的图区间上发现了一个QTL，解释了16%的表型变异，LOD值为3.09。另外还检测到两个不显著的QTL，分别位于LG 3的CHI-ASB 1区间（LOD=2.04）和LG 7的IPI-FLC 3区间（LOD=2.25）。定位到LG 2和LG 9上的QTL可用于抗病育种的分子标记辅助选择。
For quantitative trait loci (QTL) controlling resistance to Xanthomonas campestris pv. Campestris, we constructed linkage map using cleaved amplified plymorphic sequences (CAPS) and sequence-related amplified polymorphism (SRAP) analysis with disease rating of F3 families obtained from a susceptible broccoli and resistant cabbage [Green commet P09 * Reiho P01]. We established inoculation technique. In this technique, leaves from approximately 50-day old F3 plants were inoculated by cutting 1.0 cm at mid vain near the margins. A total of 38 CAPS and 60 SRAP primer pairs were screened to assess parental polymorphism against black rot resistance. Ninety two markers were distributed in 10 linkage groups (LGs) covering 320.5 cM (centimorgan), with average 3.56 cM interval between markers. Two genomic regions on LG 2 and LG 9 were significantly associated with resistance to the disease. The analysis revealed QTLs in the map interval between CAM1-GSA1 on LG 2 accounting for up to 10% of the phenotypic variation and one QTL in the map interval between F12-R12e-BORED on LG 9 explaining 16% phenotypic variation with LOD score of 3.09. Two additional non-significant QTLs on LG 3 in the interval between CHI-ASB1 (LOD=2.04) and on LG 7 in the interval between IPI-FLC3 (LOD=2.25) were also detected for resistance to the disease. The QTLs, which were mapped to LG 2 and LG 9 for the disease, could be useful for marker-assisted selection in resistance breeding.