Overestimation of Incidence of Hepatitis B Virus Mixed-Genotype Infections by Use of the New Line Probe INNO-LiPA Genotyping Assay
Overestimation of Incidence of Hepatitis B Virus Mixed-Genotype Infections by Use of the New Line Probe INNO-LiPA Genotyping Assay
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DOI：
10.1128/jcm.01888-10
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发表时间：
2011-03-01
影响因子：
9.4
通讯作者：
Servant-Delmas, Annabelle
中科院分区：
文献类型：
--
作者：
Mercier, Melanie;Laperche, Syria;Servant-Delmas, Annabelle
新版本的INNO-LiPA HBV基因分型检测试剂盒（Innogenetics）开发用于识别所有B型肝炎病毒（HBV）基因型，A至H，已进行了评价，与200份样本克隆前或克隆后PCR扩增的HBV DNA测序进行了比较。INNO-LiPA获得的基因分型数据与两种方法表征的179个样本中直接测序的数据一致。INNO-LiPA显示28例混合感染。然而，分子克隆后的测序仅证实了其中的15个，并且没有鉴定出任何基因型H（n = 9）。我们的研究表明，INNO-LiPA高估了混合感染的结果，错误的基因型H检测。
The new version of the INNO-LiPA HBV genotyping assay (Innogenetics) developed to identify all hepatitis B virus (HBV) genotypes, A to H, has been evaluated in comparison with sequencing of PCR-amplified HBV DNA from 200 samples before or after cloning. The genotyping data obtained with INNO-LiPA were in agreement with those from direct sequencing in the 179 samples characterized by the two methods. INNO-LiPA revealed 28 mixed infections. However, sequencing after molecular cloning confirmed only 15 of them and did not identify any that were of genotype H (n = 9). Our study demonstrates that INNO-LiPA overestimates mixed infections as a result of erroneous genotype H detection.