Immunomodulatory activity-guided isolation and characterization of a novel polysaccharide from Atractylodis macrocephalae Koidz.
Immunomodulatory activity-guided isolation and characterization of a novel polysaccharide from Atractylodis macrocephalae Koidz.
复制标题
DOI：
10.1016/j.ijbiomac.2020.06.003
复制
发表时间：
2020-06
影响因子：
8.2
通讯作者：
W. Xue;Yue Gao;Qianwen Li;Qibin Lu;Zhengying Bian;Lei Tang;Yu Zeng;Cong Chen;Wei Guo
中科院分区：
文献类型：
--
作者：
W. Xue;Yue Gao;Qianwen Li;Qibin Lu;Zhengying Bian;Lei Tang;Yu Zeng;Cong Chen;Wei Guo
白术是一种传统的中草药，已被广泛和经验性地用于原因不明的复发性流产。本研究从白术中提取了一种多糖，RAMP 2的分子量为4.354 × 103Da，由甘露糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成。NMR结果表明，RAMP 2中的主要连接键为→ 3-β-glcp-（1 →，→ 3，6-β-glcp-（1 →，→ 6-β-glcp-（1 →，T-β-glcp-（1 →，→ 4-α-galpA-（1 →，→ 4-α-galpA-6-OMe-（1 →，→ 5-α-araf-（1 →，→ 4，6-β-manp-（1 →和→ 4-β-galp-（1 →）。TEM和SEM结果表明RAMP 2为球形结构。体外实验表明，RAMP2可增加Treg细胞的比例，上调Foxp3、IL-10和IL-2的mRNA表达，并促进IL-10和IL-2的分泌。RAMP2进一步显示增加Treg细胞中的STAT5磷酸化水平，表明RAMP2通过IL-2/STAT5途径增加Treg细胞的数量。
The Rhizoma ofAtractylodis macrocephalaKoidz. is a traditional Chinese herbal medicine that has been widely and empirically used for unexplained recurrent spontaneous abortion. In this study, a polysaccharide from the Rhizoma ofAtractylodis macrocephalaKoidz., designated as RAMP2, with an absolute molecular weight of 4.354 × 103Da was isolated and found to be composed of mannose, galacturonic acid, glucose, galactose and arabinose. The NMR results displayed that →3-β-glcp-(1→, →3,6-β-glcp-(1→, →6-β-glcp-(1→, T-β-glcp-(1→, →4-α-galpA-(1→, →4-α-galpA-6-OMe-(1→, →5-α-araf-(1→, →4,6-β-manp-(1→ and →4-β-galp-(1→ were the main linkages in RAMP2. TEM and SEM results indicated that RAMP2 was globular in structure. Furthermore,in vitroexperiments on murine CD4+T cells revealed that RAMP2 could increase the percentage of Treg cells, up-regulate Foxp3, IL-10 and IL-2 mRNA expressions and the secretion of IL-10 and IL-2. RAMP2 was further shown to increase STAT5 phosphorylation levels in Treg cells, suggesting that RAMP2 increased the number of Treg cells through IL-2/STAT5 pathway.