Comparative meta-proteomic analysis for the identification of novel plasmodesmata proteins and regulatory cues
Comparative meta-proteomic analysis for the identification of novel plasmodesmata proteins and regulatory cues
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DOI：
10.1101/2021.05.04.442592
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发表时间：
2021-05
期刊：
影响因子：
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通讯作者：
P. Kirk;Sam Amsbury;Liam German;Rocio Gaudioso-Pedraza;Yoselin Benitez-Alfonso
中科院分区：
文献类型：
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作者：
P. Kirk;Sam Amsbury;Liam German;Rocio Gaudioso-Pedraza;Yoselin Benitez-Alfonso
细胞间信号传导的主要途径是通过称为胞间连丝（PD）的细胞壁嵌入孔形成共质。PD调节植物发育和对环境的反应的许多方面，然而，我们对影响其结构和渗透性的因素的理解是有限的。在本文中，提出了一种荟萃分析作为一种工具，用于识别影响PD运输的条件和PD蛋白质组的计算机生成的物种的利益。定制的管道搜索整个基因组的蛋白质结构特征和实验蛋白质组上鉴定的保守结构域，并使用它来预测22种相容植物物种中的PD候选物。使用计算机蛋白质组和微阵列分析，PD基因和影响PD功能的条件之间的相互作用进行了鉴定。高盐度和渗透胁迫影响了大量的PD候选基因，我们提供的证据表明，这些条件下调节GFP的共质运输。使用管道，在硅PD蛋白质组的蒺藜苜蓿，作为一个例子，其中的实验数据是不可用的。候选受体样蛋白的鉴定在M.表达荧光标记蛋白融合体的蒺藜转基因根。总之，这些结果突出了我们新设计的工具在鉴定影响不同植物物种PD的新因素和蛋白质方面的作用。
A major route for cell-to-cell signaling is via cell wall-embedded pores termed plasmodesmata (PD) forming the symplasm. PD regulate many aspects of plant development and responses to the environment however, our understanding of what factors affect their structure and permeability is limited. In this paper, a meta-analysis is presented as a tool for the identification of conditions affecting PD transport and in silico generation of PD proteomes for species of interest. The custom-built pipeline searches the whole genome for protein structural features and conserved domains identified on experimental proteomes and use it to predict PD candidates in 22 compatible plant species. Using the in silico proteome and microarray analysis, interactions between PD genes and conditions affecting PD function are identified. High salinity and osmotic stress affect a significant number of PD candidate genes and we provide evidence that these conditions regulate symplasmic transport of GFP. Using the pipeline, the in silico PD proteome for Medicago truncatula was generated, as an example of a plant in which experimental data is not available. The identification of a candidate receptor like protein was experimentally validated in M. truncatula transgenic roots expressing fluorescently tagged protein fusion. Together the results highlight the power of our newly designed tool in the identification of new factors and proteins influencing PD in diverse plant species.