Optimization of the microencapsulated islet for transplantation
Optimization of the microencapsulated islet for transplantation
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DOI：
10.1006/jsre.1997.5258
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发表时间：
1998-04-01
影响因子：
2.2
通讯作者：
Opara, EC
中科院分区：
文献类型：
--
作者：
Garfinkel, MR;Harland, RC;Opara, EC
背景。分离胰岛的微囊化是一种很好的方法，可以在同种异体和异种移植物中提供针对细胞免疫反应的保护。目前的微胶囊化方法需要海藻酸盐-钙核的螯合，这会溶解胶囊的结构支撑，并可能对耐久性产生不利影响。本研究的目的是确定未进行螯合的微囊胰岛对葡萄糖刺激的体外反应。材料和方法。使用喷气注射泵液滴发生器，将从雄性 Sprague-Dawley 大鼠中分离的胰岛封装在藻酸盐中，然后用聚-L-赖氨酸、盐水和第二层藻酸盐洗涤。然后测试不同组的微囊化胰岛在螯合之前或之后对高葡萄糖灌注的反应，并将该反应与未封装的胰岛的反应进行比较。结果。螯合微囊胰岛对 16.7 mM (300 mg%) 的刺激表现出正常的双相胰岛素反应。因此，在第一阶段，胰岛素分泌从平均+/-SEM基础速率3005+/-645增加到刺激速率5165+/-1030pg/min(P<0.05，n=6)，与用未封装的胰岛获得的结果相当。相反，未螯合的微囊化胰岛对 16.7 mM 葡萄糖的刺激没有反应。然而，这些未螯合的微胶囊培养 24 小时后，观察到微小但显着的反应。结论。尽管培养未螯合的胰岛微胶囊可以增强其功能，但微胶囊核心的螯合对于封闭胰岛的最佳功能至关重要。 (C) 1998 年学术出版社。
Background. Microencapsulation of isolated islets is a good method for providing protection against immunologic reactions to the cells in both allogeneic and xenogenic grafts. Current methods of microencapsulation require chelation of the alginate-calcium core, which solubilizes the structural support of the capsules and may adversely affect durability. The purpose of the present study was to determine the in vitro response to glucose stimulation, of microencapsulated islets that have not been subjected to chelation.Materials and methods. Using an air-jet-syringe-pump droplet generator, islets isolated from male Sprague-Dawley rats were encapsulated in alginate, followed by washes with poly-l-lysine, saline, and a second coat of alginate. Different groups of the microencapsulated islets were then tested for response to high glucose perifusion, before or after chelation, and the responses were compared with those of unencapsulated islets.Results. Chelated microencapsulated islets showed a normal biphasic insulin response to stimulation with 16.7 mM (300 mg%). Thus, insulin secretion increased from a mean +/- SEM basal rate of 3005 +/- 645 to a stimulated rate of 5165 +/- 1030 pg/min (P < 0.05, n = 6) in the first phase, comparable to results obtained with the unencapsulated islets. In contrast, unchelated microencapsulated islets did not respond to stimulation with 16.7 mM glucose. However, after a 24-h culture of these unchelated microcapsules, a small but significant response was observed.Conclusions. Although culturing unchelated microcapsules of islets may enhance their function, chelation of the microcapsular core is essential for optimal function of the enclosed islets. (C) 1998 Academic Press.