Investigation of an outbreak of Campylobacter upsaliensis in day care centers in Brussels: analysis of relationships among isolates by phenotypic and genotypic typing methods.
Investigation of an outbreak of Campylobacter upsaliensis in day care centers in Brussels: analysis of relationships among isolates by phenotypic and genotypic typing methods.
复制标题
对布鲁塞尔日托中心乌普萨利弯曲杆菌爆发的调查：通过表型和基因型分型方法分析分离株之间的关系。
DOI：
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发表时间：
1995
影响因子：
6.4
通讯作者：
J. V. D. Plas
中科院分区：
文献类型：
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作者：
H. Goossens;B. Giesendorf;Peter Vandamme;L. Vlaes;C. Borre;A. Koeken;Wim Quint;W. Blomme;P. Hanicq;D. Koster;H. Hofstra;J. Butzler;J. V. D. Plas
布鲁塞尔4个日托中心（A、B-1、B-2和C）爆发的上saliensis弯曲杆菌感染了44名儿童。腹泻是主要症状。从1991年1月至1992年6月，分别从A、B-1和B-2中心的68名儿童中分离出3名、1名和21名暴发菌株，从C中心的22名儿童中分离出19名，分别用免疫印迹法对B-2和C中心10名儿童中的9名和16名儿童中的13名的血清标本检测出IgG、IgM和IgA抗体。采用生物分型、DNA限制性内切分型和药敏分型、全细胞蛋白和质粒分析、限制性内切片段长度多态性（RFLP）和聚合酶链反应（PCR）对菌株进行分型。根据RFLP和PCR分型，菌株可分为两种相关性较强的克隆变异，一种仅从A中心儿童中分离到，另一种仅从其他日托中心儿童中分离到。
An outbreak of Campylobacter upsaliensis in four Brussels day care centers (A, B-1, B-2, and C) affected 44 children. Diarrhea was the major symptom. From January 1991 to June 1992, the outbreak strain was isolated from 3, 1, and 21 (of 68) children in centers A, B-1, and B-2, respectively, and from 19 of 22 children in center C, IgG, IgM, and IgA antibodies were detected by Western blotting of serum specimens of 9 of 10 and 13 of 16 children in centers B-2 and C, respectively. Strains were typed by biotyping, DNA restriction-based and antibiotic susceptibility typing, whole cell protein and plasmid analysis, restriction fragment length polymorphism (RFLP), and polymerase chain reaction (PCR). On the basis of RFLP and PCR typing, the strains could be divided into two strongly related clonal variants: One was isolated only from the children of center A and the second only from children in the other day care centers.
影响因子：
14.9
作者：
AKOPYANZ, N;BUKANOV, NO;BERG, DE
通讯作者：
BERG, DE