Sexually dimorphic expression of multiple doublesex-related genes in the embryonic mouse gonad
Sexually dimorphic expression of multiple doublesex-related genes in the embryonic mouse gonad
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DOI：
10.1016/s1567-133x(02)00071-6
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发表时间：
2003-03-01
影响因子：
1.2
通讯作者：
Zarkower, D
中科院分区：
文献类型：
--
作者：
Kim, SS;Kettlewell, JR;Zarkower, D
迄今为止，不同门的性调节基因的唯一分子相似性包括果蝇的doublemex（dsx），秀丽隐杆线虫的mAb-3和mAb-23，以及脊椎动物的Dmrt 1。这些基因编码DM结构域转录因子（DM = dsx和mab-3），并且是性分化所需的。在dsx和mAb-3的情况下，这两个基因控制性发育的类似方面，结合类似的DNA序列，并且能够在体内进行功能取代。所有三个门都有多个DM结构域基因，但不知道其中有多少涉及性发育。例如，哺乳动物至少有7个DM结构域基因，但胚胎表达仅详细检查了Dmrt 1（dsx和mAb-3相关转录因子1）。我们已经确定了额外的小鼠DM结构域基因，并研究了它们在小鼠胚胎中的表达，重点是发育中的性腺。除Dmrt 1外，至少有三种鼠DM结构域基因在胚胎性腺中表达：Dmrt 4在两种性别的性腺中以相似的水平表达; Dmrt 3在雄性中表达更高; Dmrt 7在雌性中表达更高。其他三个基因在胚胎性腺中的表达很低或不存在。其中两个，Dmrt 5和Dmrt 6，主要在大脑中表达，第三个，Dmrt 2，在前体中胚层和发育中的体节中表达。我们的数据表明，多个DM结构域基因可能参与哺乳动物的性发育，并且它们可能在睾丸和卵巢发育中起作用。(C)2002 Elsevier Science B. V.保留所有权利。
The only molecular similarity shown so far for sexual regulatory genes among different phyla involves doublesex (dsx) of Drosophila, mab-3 and mab-23 of Caenorhabditis elegans, and Dmrt1 of vertebrates. These genes encode DM domain transcription factors (DM = dsx and mab-3) and are required for sexual differentiation. In the case of dsx and mab-3, the two genes control analogous aspects of sexual development, bind similar DNA sequences, and are capable of functional substitution in vivo. All three phyla have multiple DM domain genes, but it is unknown how many of these are involved in sexual development. Mammals, for example, have at least seven DM domain genes, but embryonic expression has only been examined in detail for Dmrt1(dsx- and mab-3 related transcription factor 1). We have identified additional murine DM domain genes and have examined their expression in the mouse embryo, with emphasis on the developing gonad. At least three murine DM domain genes in addition to Dmrt1 are expressed in the embryonic gonad: Dmrt4 is expressed at similar levels in gonads of both sexes Dmrt3 is more highly expressed in males; and Dmrt7 is more highly expressed in females. Expression of three other genes is low or absent in the embryonic gonad. Two of these, Dmrt5 and Dmrt6, are expressed primarily in the brain, and the third, Dmrt2, is expressed in presomitic mesoderm and developing somites. Our data suggest that multiple DM domain genes may be involved in mammalian sexual development, and that they may function in both testis and ovary development. (C) 2002 Elsevier Science B.V. All rights reserved.