Use and abuse of the quasi-steady-state approximation
Use and abuse of the quasi-steady-state approximation
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DOI：
10.1049/ip-syb:20050104
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发表时间：
2006-07-01
期刊：
影响因子：
--
通讯作者：
Schnell, S.
中科院分区：
文献类型：
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作者：
Flach, E. H.;Schnell, S.
研究了开放的单酶、单底物反应的瞬态动力学行为。该反应遵循货车Slyke-Cullen机理，这是Michaelis-Menten反应的一个特例。进行分析和不应用准稳态近似。整个系统的分析表明，生化途径耦合，产生持续的振荡行为的酶反应的条件。简化模型没有表现出这种行为。该结果对开放生化反应的分析和代谢系统的建模具有重要意义。
The transient kinetic behaviour of an open single enzyme, single substrate reaction is examined. The reaction follows the Van Slyke-Cullen mechanism, a spacial case of the Michaelis - Menten reaction. The analysis is performed both with and without applying the quasi-steady-state approximation. The analysis of the full system shows conditions for biochemical pathway coupling, which yield sustained oscillatory behaviour in the enzyme reaction. The reduced model does not demonstrate this behaviour. The results have important implications in the analysis of open biochemical reactions and the modelling of metabolic systems.