The expression of CD44v6 in colon: from normal to malignant
The expression of CD44v6 in colon: from normal to malignant
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DOI：
10.1016/j.anndiagpath.2015.10.010
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发表时间：
2016-02-01
影响因子：
2
通讯作者：
Jess, Heidi
中科院分区：
文献类型：
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作者：
Afify, Alaa;Durbin-Johnson, Blythe;Jess, Heidi
CD44v6是一种完整的跨膜蛋白，属于粘附分子受体家族，在肿瘤的生长、进展和转移中起重要作用。本研究的目的是评估CD44v6在正常、增生、腺瘤和恶性结肠上皮中的表达，并确定其与肿瘤病理分期和淋巴结转移的关系。我们检测了CD44v6在正常结肠组织（n = 25）、增生性息肉（n = 45）、管状腺瘤（n = 57）、管状绒毛状腺瘤（n = 25）、绒毛状腺瘤（n = 9）、I期腺癌（n = 26）、III期腺癌（n = 26）和淋巴结转移（n = 26）中的免疫组化表达。对阳性细胞百分比和染色强度进行评估和评分。采用logistic回归和McNemar检验进行统计学分析。所有正常结肠组织和增生性息肉均显示局限于隐窝底部的CD44v6染色。在管状腺瘤中，49例（86%）表面腺瘤上皮增生异常表达CD44v6。CD44v6表达于管状绒毛腺瘤的腺区21例（84%），绒毛部分18例（72%）。所有绒毛腺瘤均表达CD44v6。CD44v6在23例（88%）I期腺癌、24例（92%）III期腺癌和9例（35%）转移性腺癌中表达。我们得出结论，CD44v6在癌前和恶性结肠病变中的表达增加表明CD44v6可能在功能上参与了腺瘤向癌的进展。CD44v6与肿瘤病理分期无关，在转移性肿瘤细胞获得迁移功能的过程中丢失。(C) 2015爱思唯尔公司版权所有。
CD44v6, an integral transmembrane protein belonging to a family of adhesion molecule receptors, plays an important role in tumor growth, progression and metastasis. The purpose of this study was to evaluate the expression of CD44v6 in normal, hyperplastic, adenomatous, and malignant colonic epithelium and to determine its correlation with tumor pathologic stage and lymph node metastasis. We examined the immunohistochemical expression of CD44v6 in normal colonic tissue (n = 25), hyperplastic polyps (n = 45), tubular adenomas (n = 57), tubulovillous adenomas (n = 25), villous adenomas (n = 9), adenocarcinomas stage I (n = 26), adenocarcinomas stage III (n = 26), and lymph node metastasis (n = 26). The percentage of positive cells and the staining intensity were assessed and scored. Statistical analysis was performed using logistic regression and McNemar test. All normal colonic tissue and hyperplastic polyps showed CD44v6 staining confined to the base of the crypt. In tubular adenomas, the dysplastic surface adenomatous epithelium expressed CD44v6 in 49 (86%) cases. CD44v6 was expressed in the glandular areas of tubulovillous adenomas in 21 (84%) cases and in the villous portion in 18 (72%) cases. All villous adenomas expressed CD44v6. CD44v6 was expressed in 23 (88%) cases of stage I adenocarcinomas, in 24 (92%) cases of stage III adenocarcinomas, and in 9 (35%) cases of metastatic adenocarcinomas. We concluded that the gain of CD44v6 expression in premalignant and malignant colonic lesions suggests that CD44v6 may be functionally involved in the adenoma-to-carcinoma progression. CD44v6 did not correlate to tumor pathologic stage and is lost during the acquisition of migratory function by metastatic tumor cells. (C) 2015 Elsevier Inc. All rights reserved.