Positive charge of chitosan retards blood coagulation on chitosan films
Positive charge of chitosan retards blood coagulation on chitosan films
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DOI：
10.1177/0885328211432487
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发表时间：
2013-05-01
影响因子：
2.9
通讯作者：
Zhang, Xiufang
中科院分区：
文献类型：
--
作者：
He, Qing;Gong, Kai;Zhang, Xiufang
在本研究中，采用NaOH水溶液或乙醇溶液脱酸制备了一系列不同质子化度的壳聚糖薄膜。然后用这些膜作为模型来研究壳聚糖的正电荷对血液凝固的影响。结果表明，壳聚糖的正电荷是一把双刃剑，它促进红细胞粘附、纤维蛋白原吸附和血小板粘附和活化，但抑制接触系统的活化。与主流观点相反，我们发现壳聚糖的正电荷延缓了这些膜上凝血酶的产生和血液凝固。造成这种现象的原因至少有两个。首先，正电荷抑制接触活化；其次，在凝血早期，正电荷不能显著促进散装期非粘附血小板的活化。本研究结果有助于进一步了解壳聚糖膜上血液凝固的机理，为今后开发新型壳聚糖止血装置提供参考。
In this study, a series of chitosan films with different protonation degrees were prepared by deacidification with NaOH aqueous or ethanol solutions. The films were then used as a model to investigate the effects of the positive charge of chitosan on blood coagulation. The results showed that the positive charge of chitosan acted as a double-edged sword, in that it promoted erythrocyte adhesion, fibrinogen adsorption, and platelet adhesion and activation, but inhibited activation of the contact system. In contrast to prevailing views, we found that the positive charge of chitosan retarded thrombin generation and blood coagulation on these films. At least two reasons were responsible for this phenomenon. First, the positive charge inhibited the contact activation, and second, the positive charge could not significantly promote the activation of non-adherent platelets in the bulk phase during the early stage of coagulation. The present findings improve our understanding of the events leading to blood coagulation on chitosan films, which will be useful for the future development of novel chitosan-based hemostatic devices.