Regulation of phosphatidylinositol 4-phosphate 5-kinase activity by partner proteins.
Regulation of phosphatidylinositol 4-phosphate 5-kinase activity by partner proteins.
复制标题
伴侣蛋白调节磷脂酰肌醇 4-磷酸 5-激酶活性。
DOI：
10.1016/s0076-6879(07)34009-3
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发表时间：
2007
影响因子：
--
通讯作者：
Yokozeki,Takeaki
中科院分区：
文献类型：
--
作者：
Kanaho,Yasunori;Nakayama,Kazuhisa;Frohman,MichaelA;Yokozeki,Takeaki
磷脂酰肌醇4，5-二磷酸[PI（4，5）P（2）]是一种用途广泛的磷脂，在信号转导、肌动蛋白细胞骨架重组、网格蛋白依赖的内吞作用和膜形态调节中起着重要作用。在哺乳动物细胞中，PI（4，5）P（2）主要由磷脂酰肌醇4-磷酸[PI（4）P] 5-激酶（PIP 5 K）通过肌醇环D-5位的PI（4）P磷酸化合成。PIP 5 K由PIP 5 K α、β和γ三种亚型以及γ同工酶的三种剪接变体组成。虽然PIP 5 K γ剪接变体PIP 5 K γ 661似乎被在粘着斑形成中起关键作用的talin和接头复合物AP-2非常特异性地激活，但目前对其他PIP 5 K同工酶活性的调节尚未完全了解。为了了解每种PIP 5 K同工酶的激活机制和特异性生理功能，需要鉴定每种PIP 5 K同工酶的特异性激活剂。本章介绍了常用的测定方法，用于测量相互作用和激活的PIP 5 K同工酶的激活剂，迄今确定。此外，程序制备的PIP 5 K同工酶和激活剂进行了描述。
The remarkably versatile phospholipid, phosphatidylinositol 4, 5-bisphosphate [PI (4, 5) P (2)], plays crucial roles in signal transduction, actin cytoskeleton reorganization, clathrin-dependent endocytosis, and regulation of membrane morphology. In mammalian cells, PI (4, 5) P (2) is synthesized predominantly by phosphatidylinositol 4-phosphate [PI (4) P] 5-kinase (PIP5K) through phosphorylation of PI (4) P at the D-5 position of the inositol ring. PIP5K is composed of three isoforms, PIP5Kalpha, beta, and gamma, and three splicing variants of the gamma isozyme. Although the PIP5Kgamma splicing variant PIP5Kgamma661 appears to be very specifically activated by talin, which plays a crucial role in focal adhesion formation, and the adaptor complex AP-2, the regulation of activities of other PIP5K isozymes is not fully understood at present. To understand the activation mechanism and the physiological function specific to each PIP5K isozyme, it is required to identify a specific activator of each PIP5K isozyme. This chapter describes common assays used to measure interaction and activation of PIP5K isozymes by activators thus far identified. In addition, procedures for preparation of PIP5K isozymes and activators are described.